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目的:通过对儿童白血病患者血浆循环DNA定量检测,分析血浆循环DNA含量用于儿童白血病诊断的价值。方法:用血液微量DNA抽提试剂盒提取血浆循环DNA,以SYBR Green I荧光染色法分别检测46例儿童急性白血病患者、20例健康对照标本血浆循环DNA含量;利用受试者工作特征(ROC)曲线评价血浆循环DNA对儿童白血病的诊断价值。结果:儿童白血病患者血浆循环DNA含量为(30.04±15.13)ng/mL,高于对照组(12.22±7.10)ng/mL,二者差异有统计学意义(P<0.05);循环DNA含量16.31ng/mL为诊断儿童白血病最佳临界值,敏感性和特异度分别为86.96%和75.00%,ROC曲线下面积(AUC)为0.879。结论:血浆循环DNA定量检测有可能成为一种新的用于儿童白血病诊断方法。
OBJECTIVE: To evaluate the value of plasma DNA content in the diagnosis of pediatric leukemia by quantitative detection of plasma circulating DNA in childhood leukemia patients. Methods: Circulating DNA was extracted from plasma using DNA micro-extraction kit. The contents of circulating DNA in 46 children with acute leukemia and 20 healthy controls were detected by SYBR Green I fluorescent staining. The receiver operating characteristic (ROC) Curvilinear evaluation of plasma circulating DNA in the diagnosis of childhood leukemia. Results: The content of circulating DNA in children with childhood leukemia was (30.04 ± 15.13) ng / mL, which was significantly higher than that in the control group (12.22 ± 7.10) ng / mL, the difference was statistically significant (P <0.05) / mL for the diagnosis of childhood leukemia the best cut-off value, sensitivity and specificity were 86.96% and 75.00%, the area under the ROC curve (AUC) was 0.879. Conclusion: Quantitative detection of plasma circulating DNA may become a new diagnostic method for childhood leukemia.