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目的 :了解糖尿病膈肌微量元素与氧化应激的关系。方法 :采用原子吸收光谱法 ,测定糖尿病 2 8d大鼠膈肌组织中铜 (Cu)、锰 (Mn)锌 (Zn)、铁 (Fe)、镉 (Cd)、铬 (Cr)、钴 (Co)、钼 (Mo)和锂 (Li)的含量 ,并同时测定超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果 :糖尿病膈肌Cu、Zn、Cr、Li含量明显低于对照组 ,Fe和Cd明显高于对照组 ,而Mn、Co、Mo与对照组无明显差别。糖尿病膈肌组织SOD活性明显低于对照组 ,MDA含量明显高于对照组。糖尿病膈肌组织Zn含量与其SOD活性呈正相关 ,与MDA含量呈负相关 ,Cd含量与SOD活性呈负相关。结论 :糖尿病 2 8d膈肌组织存在Cu、Zn、Fe、Cd、Cr、Li含量的变化及氧化应激增加 ,Zn和Cd在糖尿病膈肌组织氧化应激变化中具有重要意义。
Objective: To understand the relationship between trace elements in diaphragmatic diaphragms and oxidative stress. Methods: The contents of Cu, Mn, Zn, Fe, Cd, Cr and Cr in diaphragmatic muscle of diabetic rats were measured by atomic absorption spectrometry , Molybdenum (Mo) and lithium (Li), and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were determined simultaneously. Results: The contents of Cu, Zn, Cr and Li in diaphragmatic muscle of diabetic group were significantly lower than those in control group, and the contents of Fe and Cd in diaphragmatic muscle were significantly higher than those in control group. There was no significant difference between Mn, Co, Mo and control group. Diabetic diabetic diaphragm activity was significantly lower than the control group, MDA content was significantly higher than the control group. The content of Zn in diabetic diaphragmatic tissue was positively correlated with its SOD activity, negatively correlated with MDA content, and the content of Cd was negatively correlated with SOD activity. CONCLUSION: The content of Cu, Zn, Fe, Cd, Cr and Li in diaphragmatic muscle tissue of diabetic rats increases and the oxidative stress increases. Zn and Cd are important in the changes of diaphragmatic muscle oxidative stress.