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目的探讨肝细胞不典型增生细胞核ＤＮＡ含量与甲胎蛋白、癌胚抗原及铁蛋白３种肿瘤标志表达之间的关系。方法采用免疫组织化学ＰＡＰ法及显微分光光度计检测２２例肝细胞不典型增生甲胎蛋白、癌胚抗原和铁蛋白的表达情况和细胞核ＤＮＡ含量。结果肝细胞不典型增生的甲胎蛋白、癌胚抗原表达与非不典型增生和肝癌细胞不同，而铁蛋白的表达在三者之间无明显差别。细胞核ＤＮＡ含量及分布介于癌细胞与非不典型增生细胞之间（Ｐ＜０．０５）。结论肝细胞不典型增生细胞核ＤＮＡ含量的变化与甲胎蛋白、癌胚抗原的表达存在一定的关系，提示肝细胞不典型增生是一类增生性病变，其中含非整倍体较多的可能是一种癌前细胞。
Objective To investigate the relationship between nuclear DNA content in atypical hyperplasia of hepatocytes and expression of three tumor markers: alpha fetoprotein, carcinoembryonic antigen and ferritin. Methods The expression of alpha-fetoprotein, carcinoembryonic antigen and ferritin and the nuclear DNA content in 22 cases of atypical hyperplasia of hepatocytes were detected by immunohistochemical PAP and microspectrophotometer. Results The expression of alpha fetoprotein and carcinoembryonic antigen in atypical hyperplasia of hepatocytes was different from that in non-atypical hyperplasia and hepatocellular carcinoma cells. There was no significant difference in the expression of ferritin between the three groups. Nuclear DNA content and distribution were between the cancer cells and non-atypical hyperplasia cells (P<0.05). Conclusion There is a certain relationship between the changes of nuclear DNA content in dysplasia of hepatocytes and the expression of alpha fetoprotein and carcinoembryonic antigen, suggesting that atypical hyperplasia of hepatocytes is a type of proliferative lesions, and more may be involved in aneuploidy A precancerous cell.