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目的 研究恶性滋养细胞肿瘤分泌的人绒毛膜促性腺激素（ｈＣＧ） 中的异常糖链结构应用于临床诊断的可能性。 方法 测定２２ 例正常孕妇（ＮＰ） 、１２ 例良性葡萄胎（ＨＭ） 、１８ 例侵蚀性葡萄胎（ＩＨＭ） 和１７ 例绒毛膜癌（ＣＣ） 患者一次性晨尿中ｈＣＧ 的含量及其在蔓陀罗凝集素（ＤＳＡ） 亲和柱上的结合率。 结果 滋养细胞疾病病人一次性尿中的ｈＣＧ 含量与ＮＰ 没有差别。ＨＭ 尿中ｈＣＧ 的ＤＳＡ 结合率与ＮＰ 无异，而ＩＨＭ 与ＣＣ 尿中ｈＣＧ 的ＤＳＡ 结合率则明显高于ＮＰ或ＨＭ，ＩＨＭＣＣ和ＮＰＨＭ 两组间没有交叉重叠。ＣＣ尿中ｈＣＧ 的ＤＳＡ 结合率更高于ＩＨＭ 组，但无统计学差别。ＩＨＭ 或ＣＣ 经化疗后，尿中ｈＣＧ 含量降低，但ｈＣＧ的ＤＳＡ 结合率的降低在统计学上较含量降低更为显著。 结论 尿ｈＣＧ 的ＤＳＡ 结合率可用于恶性滋养细胞疾病的诊断及疗效认定。
Objective To investigate the possibility of clinical diagnosis of the abnormal sugar chain structure in human chorionic gonadotropin (hCG) secreted by malignant trophoblastic tumors. Methods The content of hCG in morning urine of 22 normal pregnant women (NP), 12 cases of benign hydatidiform mole (HM), 18 cases of invasive hydatidiform mole (IHM) and 17 cases of choriocarcinoma (CC) Binding rate on DSA affinity column. Results The trophoblastic disease patients had no difference in the content of hCG in one-time urine with NP. DSA binding rate of hCG in urine was no different from that of NP, while the binding rate of DSA to hCG in IHM and CC urine was significantly higher than that of NP or HM. There was no overlap between IHMCC and NPHM. The DSA binding rate of hCG in CC urine was higher than IHM group, but there was no statistical difference. After chemotherapy, IHC or CC decreased urinary hCG levels, but the reduction in hCG DSA binding rates was statistically more significant than the decrease in levels. Conclusions The DSA binding rate of urinary hCG can be used to diagnose and treat malignant trophoblastic diseases.