利用发根农杆菌介导的转化方法,获得了大豆DAD基因在根组织中沉默的嵌合植株。同时在pHells Gate12载体基础上插入一个GFP表达盒使得对转基因组织的筛选变得更加简便;通过对荧光组织进行q PCR检测发现转基因组织与对照相比,DAD基因转录水平降低45%~70%。以上结果表明大豆DAD基因在转基因根组织中已被成功沉默,并且利用改造后的p Hells Gate12:GFP载体通过荧光可以对转基因组织进行初步快速筛选。 Using Agrobacterium rhizogenes-mediated transformation, a chimeric plant with Soybean DAD gene silencing in root tissue was obtained. At the same time, inserting a GFP expression cassette based on pHells Gate12 vector makes the selection of transgenic tissues easier. By q PCR detection of fluorescent tissues, the DAD gene transcription level is reduced by 45% -70% compared with the control. The above results indicate that Soybean DAD gene has been successfully silenced in transgenic root tissue, and the transgenic tissue can be preliminarily screened by fluorescence using the modified p Hells Gate12: GFP vector.