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目的考察磷酸二氢钾-氢氧化钠(KH_2PO_4-NaOH)缓冲溶液改良川芎Cd污染土壤后,对收获期川芎药材整体质量的影响。方法在川芎生长周期内,对其栽培土壤定期浇灌农用水及不同p H梯度的KH_2PO_4-NaOH缓冲溶液,采集收获期川芎根茎,测定其HPLC色谱图与阿魏酸含量,并与标准药材和市购川芎药材进行比较。结果与PHCK空白组及川芎标准药材相比,KH_2PO_4-NaOH缓冲液处理组川芎药材色谱图相似度均在0.90以上,相似性较好,且川芎阿魏酸含量升高,并均超过市购3批川芎药材。相对于PHCK组,PH1、PH2、PH3组阿魏酸含量分别升高了13.04%,2.06%,14.35%。结论施用KH_2PO_4-NaOH缓冲液,在修复Cd污染土壤,降低川芎药材Cd含量的同时并未对川芎药材整体质量产生显著影响,且在一定程度上其整体质量优于市场一般川芎药材。
Objective To investigate the effect of potassium dihydrogen phosphate - sodium hydroxide (KH_2PO_4-NaOH) buffer solution on improving the quality of Rhizoma Chuanxiong in the harvesting stage after improving Cd-contaminated soil of Chuanxiong Rhizoma. Methods Rhizoma Chuanxiong rhizomes were harvested from the cultivated soils periodically irrigated with agricultural water and KH 2 PO 4-NaOH buffer solutions with different pH gradient. The HPLC chromatograms and ferulic acid contents of the rhizomes were collected and compared with the standard medicinal materials and municipal Buy Chuanxiong herbs for comparison. Results Compared with the PHCK blank group and the standard medicines of Chuanxiong, the chromatograms of the Chuanxiong medicines in the KH_2PO_4-NaOH buffer treatment group were all above 0.90, the similarity was good, and the content of ferulic acid in Chuanxiong rhizome increased, Batch Chuanxiong herbs. Compared with PHCK group, the content of ferulic acid in PH1, PH2 and PH3 groups increased by 13.04%, 2.06% and 14.35% respectively. Conclusion KH_2PO_4-NaOH buffer solution can reduce the Cd content of Chuanxiong rhizome and restore the contents of Cd in Chuanxiong rhizome while not affecting the overall quality of Rhizoma Chuanxiong. To a certain extent, its overall quality is superior to that of Rhizoma Chuanxiong.