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目的研究石榴皮乙醇提取液的体外抗菌作用和毒性。方法采用抑菌环试验和营养肉汤稀释法以及动物试验法进行了实验室观察。结果含石榴皮生药150 g/L的乙醇提取液对结核分枝杆菌、蜡状芽孢杆菌、肺炎克雷伯菌等临床分离菌株和枯草杆菌黑色变种、金黄色葡萄球菌等标准菌株的抑菌环直径均大于7 mm。石榴皮乙醇提取物对白色念珠菌的最低抑菌浓度为25 g/L。小鼠急性经口LD50值大于5000 mg/kg,石榴皮提取原液对家兔皮肤刺激试验指数为0.13,属于轻度刺激性。结论石榴皮乙醇提取液具有良好的体外抗菌作用,属无毒级物质,皮肤刺激性较轻。
Objective To study the antibacterial activity and toxicity of pomegranate peel ethanol extract in vitro. Methods The antibacterial ring test and nutrient broth dilution method as well as animal experiments were conducted in laboratory. Results The ethanol extract containing 150 g / L of Pomegranate skin crude drug inhibited the growth of Mycobacterium tuberculosis, Bacillus cereus, Klebsiella pneumoniae and other clinical isolates and Bacillus subtilis black variant, Staphylococcus aureus and other standard strains of antibacterial ring Diameter greater than 7 mm. Pomegranate peel ethanol extract against Candida albicans minimum inhibitory concentration of 25 g / L. Mice oral acute LD50 value is greater than 5000 mg / kg, skin pomegranate extract extract of rabbit skin irritation test index of 0.13, are mildly irritating. Conclusion Pomegranate peel ethanol extract has a good antibacterial effect in vitro, which is a non-toxic substances, skin irritation lighter.