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目的探讨高强度聚焦超声（ＨＩＦＵ）非侵入性切除肝脏组织的剂量－效应关系及机体反应，旨在为体内深部组织肿瘤的无创性治疗提供实验基础。方法采用重庆医科大学医学超声工程研究所自行设计制造的ＧＬ－１型治疗样机（１．６ＭＨｚ，１３１５３Ｗ／ｃｍ２，３ｓ），对２８只小型香猪肝脏组织进行定位损伤。于处理前１天、处理后１天、７天、１４天、２１天、２８天测定体温、体重，抽血查肝肾功能。ＨＩＦＵ后３天解剖，对肝损伤区进行体积测定和病理学检查。结果在４０ｍｍ深度范围内，ＨＩＦＵ可体外有效地定位损伤肝脏组织。辐照时间与肝组织凝固坏死体积的关系为（１２．５±５．９）ｓ／ｃｍ３。机体反应：体温在处理后１天体温升高２．２９℃，２周内体温升高１℃，２周后恢复正常。体重治疗前后无明显变化。处理前后肝肾功能无明显异常（Ｐ＞０．０５）。副作用：皮肤损伤４例（１４．２８％），３～７天愈合；胃损伤２例（７．１４％），禁食２天，自然进食，生存良好。结论应用高强度聚焦超声非侵入性破坏香猪肝脏组织是有效的，此技术对非侵入性局部破坏人体实体瘤将有重要的临床应用前景。
Objective To investigate the dose-response relationship and body response of non-invasively resected liver tissue in high-intensity focused ultrasound (HIFU) in order to provide an experimental basis for noninvasive treatment of deep-tissue tumors in vivo. Methods The GL-1 treatment prototype (1.6MHz, 13153W / cm2, 3s) designed and manufactured by Institute of Medical Ultrasound Engineering of Chongqing Medical University was used to locate and injure the liver tissue of 28 small swine. One day before treatment, one day after treatment, seven days, 14 days, 21 days, 28 days to determine body temperature, body weight, blood test liver and kidney function. Three days after HIFU dissection, the liver injury area volume determination and pathological examination. Results Within the depth of 40 mm, HIFU effectively localized damaged liver tissue in vitro. The relationship between irradiation time and coagulation necrosis volume of liver tissue was (12.5 ± 5.9) s / cm3. Body response: body temperature increased by 1.29 ℃ at 1 day after treatment, body temperature increased by 1 ℃ within 2 weeks and returned to normal after 2 weeks. Weight before and after treatment no significant change. Liver and kidney function before and after treatment showed no abnormalities (P> 0.05). Side effects: skin injury in 4 cases (14.28%), healing in 3 to 7 days; gastric injury in 2 cases (7.14%), fasting for 2 days, natural eating, good survival. Conclusion High-intensity focused ultrasound is effective in noninvasively destroying Xiang pig’s liver tissue. This technique will have important clinical application prospects for noninvasive localized destruction of human solid tumors.