目的采用CT增强及病理学方法评估新型温敏纳米凝胶(PIB)经肝动脉栓塞治疗兔肝VX2肿瘤的效果,并探讨其作为血管栓塞剂的可行性。方法 45只健康日本大耳白兔,肝脏左叶植入VX2肿瘤组织块建模,将建模成功的荷瘤兔随机分为三组,PIB组,碘油组(DY组)及生理盐水组(NS组)各15只行经肝动脉栓塞治疗,于术前1天、术后3天、7天、14天时间点检测各组实验兔肝功能,同时行CT增强扫描,测量栓塞前后各组肿瘤体积变化并计算肿瘤体积增长率,观察肿瘤形态学改变同时结合病理学方法验证其治疗效果。结果术前1天,PIB、DY及NS各组间肝功能差异无统计学意义(P>0.05)。兔肝VX2肿瘤经肝动脉栓塞术后3天,PIB组、DY组的肝功能(ALT、AST)较NS组明显升高。对于肝功能ALT,PIB组、DY组分别与NS组差异有显著统计学意义(P<0.05)。但PIB组及DY组组间差异无统计学意义(P>0.05);对于肝功能AST,PIB组、DY组、NS组三组间差异均有统计学意义(P<0.05)。术后7天,三组间的差异无显著统计学意义(P>0.05)。术后14天,PIB、DY及NS三组间肝功能差异与术后3天各组统计学结果相同(P<0.05)。术前1天三组肿瘤体积大小的差异无显著统计学意义;术后3天、7天及14天,PIB、DY、NS三组肿瘤体积的差异均有统计学意义(P_3<0.05;P_7<0.05;P_(14)<0.05),且分别为术后3天PIB组肿瘤体积增大明显,与DY、NS组差异均有统计学意义,术后7天NS组肿瘤体积增大明显,分别与PIB、DY组差异有统计学意义。栓塞术后7天,PIB及DY组肿瘤体积增长率均较NS组低,差异有统计学意义(P<0.05),但两组间差异无显著统计学意义(P>0.05),14天后三组间肿瘤体积及体积生长率均有显著性差异(P<0.05)。影像学上的环形强化灶均为病理切片下的肿瘤细胞组织,病理所见肿瘤范围与影像学测量大体一致。结论 PIB兔肝动脉介入栓塞治疗安全有效,可明显抑制肿瘤生长,其长期抑瘤效果较碘油佳,作为血管栓塞剂可行性好。 Objective To evaluate the effect of transcatheter hepatic VX2 tumor on hepatic arterial embolization using novel CT-enhanced and pathological methods and to explore the feasibility of using it as a vascular embolic agent. Methods Forty five healthy Japanese white rabbits were implanted with VX2 tumor in the left lobe of the liver. The successful model rabbits were randomly divided into three groups: PIB group, lipiodol group (DY group) and saline group (NS group), each of which was treated with hepatic artery embolization. The liver function of each group was detected at 1 day before surgery, 3 days, 7 days and 14 days after operation. At the same time, Tumor volume changes and calculate the volume of tumor growth rate, observe the morphological changes of the tumor combined with pathological methods to verify the treatment effect. Results There was no significant difference in liver function between PIB, DY and NS groups one day before operation (P> 0.05). Three days after hepatic artery embolization, the hepatic function (ALT, AST) in PIB group and DY group was significantly higher than that in NS group. For liver function ALT, PIB group, DY group and NS group were significantly different (P <0.05). However, there was no significant difference between PIB group and DY group (P> 0.05). There were significant differences among the three groups in AST, PIB group, DY group and NS group (P <0.05). Seven days after operation, there was no significant difference between the three groups (P> 0.05). At 14 days after operation, the difference of liver function between the three groups of PIB, DY and NS was the same as that of three days after operation (P <0.05). There was no significant difference in tumor size between the three groups before operation. The tumor volume of PIB, DY and NS groups were statistically significant at 3 days, 7 days and 14 days after operation (P 3 <0.05; P 7 P <0.05; P <(14) <0.05). The tumor volume of PIB group increased significantly on the 3rd day after operation, and the difference between DY and NS group was statistically significant. The tumor volume of NS group increased significantly on the 7th day after operation, Respectively with PIB, DY group differences were statistically significant. At 7 days after embolization, the tumor volume growth rate in both PIB and DY groups was lower than that in NS group (P <0.05), but there was no significant difference between the two groups (P> 0.05) Tumor volume and volume growth rate between groups were significantly different (P <0.05). Radiographically enhanced lesions were all pathological sections of tumor cells, pathological findings of the tumor range and imaging measurements are generally consistent. Conclusion PIB rabbit hepatic artery interventional embolization is safe and effective, which can significantly inhibit tumor growth. Its long-term anti-tumor effect is better than that of lipiodol, and it is feasible as a vascular embolization agent.