本文应用青霉素酶作为标记酶,以硝酸纤维薄膜作为载体,以碘电极作为检测手段,建立了一种测定睾丸酮敏感而特异,干扰小,安全可靠的青霉素酶标免疫碘电极分析法。该法最低检出量为3.2×10~(-8)mg/ml(1.6pg/孔),睾丸酮浓度在10~(-3)～10~(-7)mg/ml范围内与电位差值(ΔE)呈线性关系(r=0.94)。日内和日间变异系数分别小于4.2％和8.3％,回收率为96.7％～106.0％。且与RIA之间有好的相关性(r=0.998)。 In this paper, penicillinase as a marker enzyme, nitrocellulose membrane as a carrier, iodine electrode as a test means to establish a testosterone sensitive and specific, small interference, safe and reliable penicillinase enzyme immunoassay electrode method. The detection limit of this method was 3.2 × 10 -8 mg / ml (1.6 pg / well) and the testosterone concentration ranged from 10 -3 to 10 -7 mg / ml with the potential difference (ΔE) was linear (r = 0.94). The intra-day and inter-day coefficients of variation were less than 4.2% and 8.3%, respectively, and the recoveries ranged from 96.7% to 106.0%. There was a good correlation with RIA (r = 0.998).