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目的:观察三七总苷对应激大鼠胃黏膜组织中褪黑素受体(MR)表达以及含量的影响。方法:采用经典的水浸-束缚应激模型,总RNA提取产物应用RevertAid First Strand cDNA Synthesis Kit进行逆转录,PCR产物进行琼脂糖电泳,经KODAK1D Image成像系统分析,以目的基因(MT1受体)与内参(GAPDH)光密度比值表示MT1受体mRNA的相对表达水平。结果:水浸-束缚应激6 h后,所有以MT1为引物的RT-PCR产物中,均可见大小为360bp的阳性条带;模型组MR的相对含量比正常组略低,三七总苷组、雷尼替丁组与正常组和模型组比较,MR的相对含量均升高(P<0.05或P<0.01)。结论:应激大鼠胃黏膜中确实存在MT1受体mRNA的表达,三七总苷组通过上调MR表达而发挥胃黏膜保护作用,其机理可能是三七总苷促进褪黑素分泌或提高其与受体结合容量所表现的MR高表达。
Objective: To observe the effect of Panax Notoginseng on the expression and content of melatonin receptor (MR) in gastric mucosa of rats with stress. Methods: The classical model of flooding - restraint stress was used. The total RNA was extracted by reverse transcription using RevertAid First Strand cDNA Synthesis Kit. The PCR product was subjected to agarose gel electrophoresis. The target gene (MT1 receptor) was detected by KODAK1D Image imaging system. The ratio of optical density to internal reference (GAPDH) indicates the relative expression level of MT1 receptor mRNA. Results: After immersion-restraint stress for 6 h, all of the RT-PCR products with MT1 as primers showed a positive band of 360 bp in size. The relative content of MR in model group was slightly lower than that in normal group. Compared with the normal group and the model group, the relative contents of MR in ranitidine group and the ranitidine group were both increased (P <0.05 or P <0.01). Conclusion: The expression of MT1 receptor mRNA does exist in gastric mucosa of stress rats. Panax notoginseng glycosides play a protective role in gastric mucosa by up-regulating MR expression. The mechanism may be that Panax notoginseng glycoside can promote or increase the secretion of melatonin MR expression of receptor-binding capacity is high.