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为了探讨盐碱胁迫条件下鱼类渗透生理调节机制,以尼罗罗非鱼(Oreochromis niloticus)为实验材料,PCR扩增得到了Na~(+)/3HCO~(-)共转运子(NBCe1)基因c DNA部分序列,比较了单盐(盐度10、盐度15)、单碱(1.5 g/L、3 g/L Na HCO3)、盐碱混合(盐度10,碱度1.5 g/L;盐度15,碱度3 g/L)胁迫后不同时间(0 h、6 h、12 h、24 h、48 h、72 h、96 h)血清渗透压、离子浓度(Na~(+)、K~(+)、Cl–、Ca2~(+))以及鳃碳酸酐酶(CA)活性、CA与NBCe1基因m RNA表达变化。结果显示,不同胁迫条件下,血清渗透压、离子浓度、鳃组织CA酶活、CA与NBCe1基因m RNA表达变化均与胁迫强度呈正相关。随时间推移,血清渗透压、离子浓度呈现先上升后下降的变化趋势,单盐、盐碱混合组血清渗透压值较单碱组高。单盐、单碱、盐碱混合组中,NBCe1基因m RNA在鳃中均呈略微上调,但不显著(P>0.05)。单碱组和盐碱混合组鳃CA活性较单盐组高,低盐碱胁迫(盐度10,碱度1.5 g/L)下CA活性较晚达最高值;不同胁迫条件下,CA基因m RNA表达均表现上调,单碱、盐碱混合组更为显著(P<0.05),推测CA较NBCe1对体内3HCO~(-)转运作用更为显著。研究结果为尼罗罗非鱼盐碱适应生理调节提供了基础资料。
In order to investigate the mechanism of physiological regulation of fish infiltration under saline-alkali stress, Nr (+) / 3HCO ~ (-) cotransporter (NBCe1) was amplified by PCR from Oreochromis niloticus The results showed that single salt (salinity 10, salinity 15), single alkali (1.5 g / L, 3 g / L Na HCO3), salinity and alkalinity ; Salinity 15, alkalinity 3 g / L) at different times (0 h, 6 h, 12 h, 24 h, 48 h, 72 h, 96 h) , K ~ (+), Cl-, Ca2 ~ (+)), gill carbonic anhydrase (CA) activity and m RNA expression of CA and NBCe1 gene. The results showed that under different stress conditions, the changes of osmotic pressure, ion concentration, CA activity in gill tissue and m RNA expression of CA and NBCe1 gene were positively correlated with the stress intensity. Over time, serum osmolality and ion concentration showed the trend of first increasing and then decreasing. The osmotic pressure of single salt and saline mixed group was higher than that of single alkali group. In the single salt, single alkali and saline-alkali mixed groups, the m RNA of NBCe1 gene slightly increased in the gills but not significantly (P> 0.05). Compared with single salt group, the activities of CA in single alkali group and saline-alkali mixed group were higher than those in the single salt group, and the CA activity reached the highest value under low salt-alkali stress (salinity 10 and alkalinity 1.5 g / L). Under different stress conditions, CA gene m RNA expression were up-regulated, single-alkali, saline-alkali mixed group was more significant (P <0.05), suggesting that CA compared with NBCe1 3HRC ~ (-) transport in vivo more significant. The results provided the basic information for physiological adaptation of Nile tilapia to saline-alkali.