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本文选择了具有清热解毒功效的五个古典方:银翘散《温病条辨》、普济消毒饮《医方集解》、黄连解毒汤《外台秘要》、五味消毒饮《医家金鉴》及葛根芩连汤《伤寒论》进行了抗菌作用的实验研究。一、药物制备: 将原方按常规煎法制成水煎剂,每剂最后浓缩至25ml,为实验用原液。二、实验动物: 昆明杂种小鼠。三、体内实验。取小鼠随机分为治疗组、预防组及对照组。治疗组和预防组均按成人剂量50倍(等效量)灌胃给药(银翘散140g/kg;普济消毒饮67.5g/kg;黄连解毒汤25g/kg;五味消毒饮55g/kg;葛根芩连汤27.5g/kg;土霉素0.42g/kg)。观察腹腔注射细菌悬液后24hr内动物死亡情况,判断体内抗菌效果。预防组在注射菌液前一天给药;治疗组在注射菌液同时给药。
This article selects five classics that have the effect of heat-clearing and detoxifying: Yin Qiao San’s “Diseases for Diseases”, Puji Disinfection Beverage “Medical Party Settlement”, Huang Lian Jie Du Tang “Wai Tai Mi Yao” and Wuwei Disinfection Drink “Medical Family Gold” Jian Yu and Ge Gen Qilian Tang “Treatise on Febrile Diseases” conducted an experimental study of the antibacterial effect. First, the preparation of drugs: The original side by conventional decoction made of decoction, the final concentration of each agent to 25ml, the experimental solution. Second, experimental animals: Kunming hybrid mice. Third, the in vivo experiment. Mice were randomly divided into treatment group, prevention group and control group. The treatment group and prevention group were administered by gavage at an adult dose of 50 times (equivalent amount) (Yinqiaosan 140g/kg; Puji disinfectant drink 67.5g/kg; Huanglian Jiedu Decoction 25g/kg; Wuwei Disinfected Drink 55g/kg Pueraria decoction with 27.5g/kg terramycin 0.42g/kg). Observe the death of the animals within 24 hours after intraperitoneal injection of the bacterial suspension and determine the antibacterial effect in vivo. The prophylactic group was administered one day before the injection of the broth; the treatment group was administered simultaneously with the injection of the broth.