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[目的]研究S100B蛋白、髓鞘碱性蛋白(MBP)在戊四氮(PTZ)致幼鼠癫痫(EP)后其学清和不同脑区的变化,探讨癫痫发作后脑组织的损伤情况。[方法]取SD幼鼠60只,随机分为对照组及实验组。实验组腹腔注射PTZ(50mg/kg);对照组腹腔注射生理盐水。根据EP发作分级,0～1级组立即取脑;2级以上随机于发作后0、6、24和72h断头,取躯干血后分别取海马、额叶皮层和中脑。采用ELISA法测定各脑区及血清S100B蛋白和MBP值。[结果]EP发作后海马、额叶、中脑和血清S100B蛋白、MBP呈动态升高过程;各脑区和血清S100B蛋白皆于发作后24h达高峰,各脑区和血清MBP皆于发作后72h达高峰。[结论]EP发作后S100B蛋白、MBP明显升高,表明EP可造成大脑神经胶质细胞、神经髓鞘的损伤。
[Objective] To investigate the changes of serum and different brain regions of S100B protein and myelin basic protein (MBP) in rats induced by pentylenetetrazole (PTZ) and to investigate the damage of brain tissue after seizure. [Method] Sixty SD young rats were randomly divided into control group and experimental group. Experimental group intraperitoneal injection of PTZ (50mg / kg); control group intraperitoneal injection of saline. According to the grading of EP, 0 ~ 1 group immediately take the brain; 2 or more were randomized at 0, 6, 24 and 72h after the onset of decapitation, the trunk blood was taken from the hippocampus, frontal cortex and midbrain. Serum S100B protein and MBP were measured by ELISA. [Results] The levels of S100B protein and MBP in the hippocampus, frontal lobe, midbrain and serum increased dynamically after EP onset. The levels of S100B protein peaked at 24 h post-seizure in all brain regions and serum MBP, 72h reached the peak. [Conclusion] The S100B protein and MBP were significantly increased after EP attack, indicating that EP can cause injury of glial cells and nerve myelin in the brain.