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目的观察镇痛剂量吗啡鞘内泵入对大鼠细胞免疫功能的影响。方法40只雄性SD大鼠随机分为5组(n=8):假手术组(F组)、生理盐水组(NS组)、M1、M2、M3组(吗啡泵入速率分别为25、5、10μg·h-1)。除F组外各组采用改良Yaksh法进行鞘内置管,置管后5d采用Alzet泵持续泵人生理盐水或吗啡,共7d。第7天各组大鼠进行福尔马林炎性疼痛实验,根据痛级(PIS)评分评价吗啡镇痛效应,处死大鼠后计算睥脏指数并分离脾脏单个核细胞进行培养,甲基-3H胸腺嘧啶核苷掺入法检测脾T淋巴细胞增殖水平,乳酸脱氢酶释放法检测自然杀伤细胞活性。结果与NS组比较,MI、M2、M3组在福尔马林炎性疼痛第一时相和第二时相的PIS评分值、脾脏指数、脾脏T淋巴细胞增殖转化水平和NK细胞活性降低(P<0.05或0.01);与M1组比较,M2、M3组脾脏T淋巴细胞增殖转化水平及NK细胞活性降低(P<0.05);与M2组比较,M3组脾脏NK细胞活性降低(P<0.05)。结论镇痛剂量吗啡鞘内泵入可抑制大鼠细胞免疫功能,且泵入速率越大,免疫抑制效应越明显。
Objective To investigate the effects of intrathecal (p <0.05) morphine on the cellular immune function in rats. Methods Forty male Sprague-Dawley rats were randomly divided into 5 groups (n = 8): sham operation group (F group), NS group, M1, M2 and M3 groups , 10 μg · h-1). Except Group F, the rats in each group were treated with the improved Yaksh method for intrathecal catheterization. After 5 days of catheterization, saline or morphine was continuously pumped by Alzet pump for 7 days. On the 7th day, the rats in each group were subjected to the inflammatory pain test in formalin. The morphine analgesic effect was evaluated according to the grade of pain (PIS). After the rats were sacrificed, the index of visceral index was calculated and the splenic mononuclear cells were isolated and cultured. The proliferation of splenic T lymphocytes was detected by the incorporation of pyrimidine nucleoside and the activity of natural killer cells was detected by lactate dehydrogenase release assay. Results Compared with the NS group, the PIS score, spleen index, the proliferation and transformation of T lymphocytes in spleen and the activity of NK cells in MI, M2 and M3 groups were lower than those in the first and second phases of inflammatory pain in formalin (P < 0.05 or 0.01). Compared with M1 group, the proliferation and transformation of T lymphocytes in spleen and the activity of NK cells decreased in M2 and M3 groups (P <0.05). Compared with M2 group, the activity of NK cells in M3 group decreased (P <0.05). Conclusion The analgesic dose of morphine intrathecal pump can inhibit cellular immune function, and the greater the pumping rate, the more obvious immunosuppressive effect.