论文部分内容阅读
目的：探讨缺血预处置及磷酸肌酸对·ＯＨ的影响。方法：以离体大鼠心脏为模型，以水杨酸为捕捉剂，利用其与再灌注产生的羟自由基（·ＯＨ）反应生成的二羟苯甲酸（ＤＨＢＡ）为指标，用高效液相色谱（ＨＰＬＣ）法检测缺血心肌再灌注冠脉流出液和心肌组织中ＤＨＢＡ含量。结果：缺血再灌注冠脉流出液和心肌ＤＨＢＡ生成增多，与对照组相比差异非常显著，缺血预处置组与非处置组相比ＤＨＢＡ生成明显降低，而且四次预处置组低于一次预处置组；缺血前给予磷酸肌酸使ＤＨＢＡ生成明显减低，但与缺血预处置并用组无明显差异。结论：缺血预处置与磷酸肌酸可以通过减少缺血再灌注心肌·ＯＨ的生成保护心肌，但二者无协同作用，且缺血预处置有累加作用。
Objective: To investigate the effect of ischemic preconditioning and creatine phosphate on · OH. Methods: Using isolated rat heart as a model and salicylic acid as a capture reagent, dihydroxybenzoic acid (DHBA) produced by the reaction with hydroxyl radical (· OH) generated by reperfusion was used as an index. Chromatographic (HPLC) method was used to detect the content of DHBA in myocardial effusion and myocardial tissue after ischemia reperfusion. Results: Compared with the control group, there was an obvious increase in myocardial effusion and myocardial DHBA production. Compared with the control group, the ischemic preconditioning group and the non-treatment group were significantly lower in DHBA production and less in the four pre-treatment groups Pretreatment group; before administration of creatine phosphate to DHBA significantly reduced generation, but with ischemic preconditioning group with no significant difference. CONCLUSION: Ischemic preconditioning and creatine phosphate can protect the myocardium by reducing the production of myocardial · OH during ischemia / reperfusion. However, there is no synergistic effect between the two treatments and the ischemic preconditioning has an additive effect.