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以矮丛蓝莓品种“美登”为试材,采用第二代测序技术对其嫩叶转录组进行了测序及生物信息学分析,研究了矮丛蓝莓功能基因组信息,以期增加对矮丛蓝莓生长发育分子机理的了解,并为矮丛蓝莓的分子标记辅助育种奠定基础。结果表明:在获得的约1G纯净数据,共拼装了41 929条Unigenes,其中被GO数据库成功注释Unigene分别涉及到生物学过程、细胞成分及分子功能相关的共45类生理代谢功能;KOG数据库注释到19 149条Unigenes,共涉及25条代谢通路;KEGG数据库成功注释13 072条Unigenes,共涉及到5个大类、18个中类、129条代谢通路;Nr数据库成功注释30 738条Unigenes,这些Unigenes共对应32 949条蛋白质的编码区。同时,生物信息学分析还显示,全部Unigenes中,有884条编码转录因子,涉及到55个家族;2 261条编码抗性基因,涉及18个家族;检测到6 591个SSR位点,其中2碱基重复的SSR数目最多,占总SSR位点数的70%。
The second generation sequencing technique was used to sequence and bioinformatics analysis of the young leaves transcriptome using the short blueberry variety “Mei Deng ” as the material, and the functional genomic information of blueberry was studied to increase the effect on short bush Blueberry growth and development of molecular mechanism of understanding, and for the short blueberry molecular marker assisted breeding laid the foundation. The results showed that a total of 41 929 Unigenes were assembled in about 1G pure data, among which, 45 were successfully involved in the biological processes, cell components and molecular functions of Unigenes, which were annotated by GO database respectively. KOG database annotation To 19 149 Unigenes involving 25 metabolic pathways; KEGG database successfully annotated 13 072 Unigenes involving 5 major categories, 18 intermediate classes and 129 metabolic pathways; Nr database successfully annotated 30 738 Unigenes, these Unigenes corresponds to a total of 32,949 protein coding regions. At the same time, bioinformatics analysis also showed that 884 transcription factors were involved in all Unigenes, involving 55 families; 2 261 genes encoding resistance, involving 18 families; 6 591 SSR loci were detected, of which 2 The SSR number of base repeat was the largest, accounting for 70% of the total number of SSR sites.