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由江汉油田采油厂污水中分离、提纯出来的硫酸盐还原菌（ＳＲＢ）菌株，采用ＡＰＩＲＰ３８ 推荐使用的培养基生成生物膜。利用交流阻抗技术研究了生物膜与腐蚀之间的关系，细菌新陈代谢产物及腐蚀产物的分析借助于电子探针及气相色谱／ 质谱联用技术（ＧＣ／ＭＳ） 。结果表明，ＳＲＢ能够达到电极表面，且在其表面形成腐蚀产物的细菌生物膜混合层，细菌包裹在腐蚀产物中，形成局部微生境。ＧＣ／ ＭＳ及电子探针的研究表明，在此微生境中细菌新陈代谢有脂肪酸、磷铁化合物、硫铁化合物生成。生物膜的存在为ＳＲＢ对胞外物质的富集提供了良好的场所，且由于代谢产物造成的微生境的差异，促进了点蚀的形成
Sulfate-reducing bacteria (SRB) strains isolated and purified from the effluent from an oil recovery plant in Jianghan Oilfield were used to generate biofilms using the medium recommended by APIRP-38. The relationship between biofilm and corrosion was studied by AC impedance technique. The analysis of bacterial metabolites and corrosion products was performed by means of electron probe and gas chromatography / mass spectrometry (GC / MS). The results show that SRB can reach the electrode surface and form a mixed layer of bacterial biofilm with corrosion products on its surface. Bacteria are trapped in the corrosion products and form local micro-habitat. Studies on GC / MS and electron probe show that bacteria metabolize fatty acids, ferrophosphorus compounds and ferrosulphites in this microbe. The presence of biofilms provides a good site for the enrichment of extracellular material by SRBs and the formation of pitting corrosion is facilitated by differences in microbiota due to metabolites