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沙门氏菌为革兰氏阴性无芽胞杆菌,国际上已发现2500多种血清型,几乎所有的沙门氏菌都能污染食物,是人畜共患的肠道致病菌之一,主要导致胃肠炎、伤寒和副伤寒。沙门氏菌的传统检测方法如免疫学检测和表型特征检测都比较复杂,检测周期较长,灵敏度低,容易延误产品储存时间,使成本增加。目前,荧光定量聚合酶链反应(RT-PCR)是检测食品中各种细菌和病毒的主流方法,该方法检测食
Salmonella is Gram-negative bacillus, and more than 2,500 serotypes have been found in the world. Almost all of Salmonella can contaminate food and is one of the zoonotic pathogens, which causes gastroenteritis, typhoid and paratyphoid. Salmonella traditional detection methods such as immunological detection and phenotypic characteristics are more complex detection, longer detection cycle, low sensitivity, easy to delay product storage time, the cost increase. At present, fluorescent quantitative polymerase chain reaction (RT-PCR) is a mainstream method for detecting various bacteria and viruses in food. The method detects food