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目的分析不同刺激下外周血单个核细胞(PBMC)中THANK基因的表达，并克隆全长的人THANK基因。方法采用将常规方法分离的外周血单个核细胞(PBMC)培养于含100ml/L FCS的RPMI 1640中，并以不同浓度的LPS、TNF-α、IL-2、IFN-γ、PHA及PMA刺激不同时间。以RT-PCR法，分析PMBC中THANK基因的转录表达，并克隆相应的全长人THANK基因。结果RT-PCR分析表明，当用IFN-γ刺激PMBC 72h后，可诱导THANK基因明显表达;而IL-2、LPS、TNF-α、PHA和PMA则不能诱导其表达。采用PCR产物克隆的方法，克隆了人THANK基因，并经DNA序列测定证实。结论克隆得到了人THANK基因，为进一步研究THANK的功能打下了基础。
Objective To analyze the expression of THANK gene in peripheral blood mononuclear cells (PBMCs) under different stimulation and clone the full length human THANK gene. Methods Peripheral blood mononuclear cells (PBMC) isolated by routine methods were cultured in RPMI 1640 containing 100 ml / L FCS and stimulated with different concentrations of LPS, TNF-α, IL-2, IFN-γ, PHA and PMA different time. The transcriptional expression of THANK gene in PMBC was analyzed by RT-PCR, and the corresponding full-length human THANK gene was cloned. Results RT-PCR analysis showed that the expression of THANK gene was induced by IFN-γ stimulation for 72h, while IL-2, LPS, TNF-α, PHA and PMA could not induce the expression of THANK gene. The human THANK gene was cloned by PCR cloning, and confirmed by DNA sequencing. Conclusion The human THANK gene was cloned and laid the foundation for further study on the function of THANK.