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目的:研究外源性野生型p53基因对人肺腺癌细胞系GLC-82的生物学作用。方法:将含有野生型p53基因的pDOR-neo逆转录病毒载体,通过lipofectin转染GLC-82细胞,用流式细胞仪、电镜及3H-TdR掺入法,观察野生型p53基因对GLC-82细胞的生物学效应。结果:电镜观察可见,转染野生型p53基因,可使GLC-82细胞出现一些病理现象,如胞浆中有明显的空泡及线粒体肿胀;流式细胞仪分析显示,野生型p53基因可阻止GLC-82细胞周期停止于G1~S区;3H-TdR掺入试验提示,野生型p53基因能够抑制GLC-82细胞DNA的合成。结论:这些结果阐明了野生型p53基因是抑制GLC-82细胞生长的部分机理。
Objective: To study the biological effects of exogenous wild-type p53 gene on human lung adenocarcinoma cell line GLC-82. METHODS: The pDOR-neo retroviral vector containing the wild-type p53 gene was transfected into GLC-82 cells by lipofectin and observed by flow cytometry, electron microscopy, and 3H-TdR incorporation to observe the wild-type p53 gene pair GLC-82. The biological effects of the cells. RESULTS: Electron microscopy showed that transfection of wild-type p53 gene could cause some pathological phenomena in GLC-82 cells, such as obvious vacuolization and mitochondrial swelling in cytoplasm; flow cytometric analysis showed that wild-type p53 gene could prevent GLC-82 cell cycle arrested in G1-S region; 3H-TdR incorporation test suggested that wild-type p53 gene can inhibit DNA synthesis in GLC-82 cells. Conclusion: These results illustrate that the wild-type p53 gene is part of the mechanism that inhibits the growth of GLC-82 cells.