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目的：研究外源性野生型ｐ５３基因对人肺腺癌细胞系ＧＬＣ－８２的生物学作用。方法：将含有野生型ｐ５３基因的ｐＤＯＲ－ｎｅｏ逆转录病毒载体，通过ｌｉｐｏｆｅｃｔｉｎ转染ＧＬＣ－８２细胞，用流式细胞仪、电镜及３Ｈ－ＴｄＲ掺入法，观察野生型ｐ５３基因对ＧＬＣ－８２细胞的生物学效应。结果：电镜观察可见，转染野生型ｐ５３基因，可使ＧＬＣ－８２细胞出现一些病理现象，如胞浆中有明显的空泡及线粒体肿胀；流式细胞仪分析显示，野生型ｐ５３基因可阻止ＧＬＣ－８２细胞周期停止于Ｇ１～Ｓ区；３Ｈ－ＴｄＲ掺入试验提示，野生型ｐ５３基因能够抑制ＧＬＣ－８２细胞ＤＮＡ的合成。结论：这些结果阐明了野生型ｐ５３基因是抑制ＧＬＣ－８２细胞生长的部分机理。
Objective: To study the biological effects of exogenous wild-type p53 gene on human lung adenocarcinoma cell line GLC-82. METHODS: The pDOR-neo retroviral vector containing the wild-type p53 gene was transfected into GLC-82 cells by lipofectin and observed by flow cytometry, electron microscopy, and 3H-TdR incorporation to observe the wild-type p53 gene pair GLC-82. The biological effects of the cells. RESULTS: Electron microscopy showed that transfection of wild-type p53 gene could cause some pathological phenomena in GLC-82 cells, such as obvious vacuolization and mitochondrial swelling in cytoplasm; flow cytometric analysis showed that wild-type p53 gene could prevent GLC-82 cell cycle arrested in G1-S region; 3H-TdR incorporation test suggested that wild-type p53 gene can inhibit DNA synthesis in GLC-82 cells. Conclusion: These results illustrate that the wild-type p53 gene is part of the mechanism that inhibits the growth of GLC-82 cells.