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目的： 探讨凋亡相关基因ｂａｋ 在人肾上腺嗜铬细胞瘤中的表达及其对肿瘤发生的意义． 方法： 应用免疫组织化学的ＳＡＢＣ方法，和兔抗人Ｂａｋ 多克隆抗体，对２３例肾上腺嗜铬细胞瘤（其中良性１８例，恶性５例）和１０例正常肾上腺组织进行了检测． 结果： Ｂａｋ 在正常肾上腺髓质中不表达，而在良恶性嗜铬细胞瘤中表达，阳性率分别为８９％ 和４０％ ． 在良性肿瘤中Ｂａｋ 表达显著强于恶性肿瘤． 结论： Ｂａｋ 不同程度地参与了良恶性嗜铬细胞瘤中的凋亡调控，对良恶性嗜铬细胞瘤鉴别诊断的意义有待深入研究．
Objective: To investigate the expression of apoptosis-related gene bak in human adrenal pheochromocytoma and its significance for tumorigenesis. METHODS: Immunohistochemical SABC method and rabbit anti-human Bak polyclonal antibody were used to detect 23 cases of adrenal pheochromocytoma (18 cases of benign and 5 cases of malignant) and 10 cases of normal adrenal tissue. Results: Bak was not expressed in normal adrenal medulla but was expressed in benign or malignant pheochromocytoma. The positive rates were 89% and 40%, respectively. Bak expression is significantly stronger than malignant tumors in benign tumors. Conclusion: Bak is involved in the regulation of apoptosis in different grades of benign and malignant pheochromocytoma. The significance of differential diagnosis of benign and malignant pheochromocytoma needs further study.