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目的 细胞与细胞之间及细胞与基质之间的粘附作用是细胞间信号传递及多种细胞生物活动的基础 ,细胞粘附作用的检测是细胞生物学研究中重要的实验方法之一。本文对采用51Cr释放实验及3 H -TdR掺入法检测细胞粘附的方法进行了比较。方法 将铺底细胞以 0 5× 10 5 /孔的密度于 96孔培养板中铺底培养 2 4h ,待检细胞以51Cr或3 H -TdR标记 ,以 1× 10 5/孔的浓度加入96孔培养板中 ,作用 4h后轻柔洗去未粘附细胞。按相应检测系统检测51Cr及3 H -TdR标记的粘附细胞的放射强度 ,比较其灵敏度和稳定性。结果 51Cr释放实验及3 H -TdR掺入法均具有较好的敏感性和稳定性。相比较 ,3 H -TdR掺入法的敏感性和稳定性更好。这两种方法均能准确地检测细胞间的粘附现象 ,并反映其相对强弱。结论 采用同位素标记的方法 ,其敏感性和稳定性均较好 ,且干扰因素少 ,所获结果稳定可靠 ,3 H -TdR掺入法的敏感性和稳定性较51Cr释放法好。
The purpose of cells and cells and cell adhesion between the matrix is the basis of cell signal transduction and a variety of cellular biological activity, cell adhesion assay is one of the important experimental methods in cell biology. In this paper, 51Cr release experiments and 3 H-TdR incorporation assay for cell adhesion were compared. Methods The basal cells were seeded on a 96-well plate at a density of 0 5 × 10 5 / well for 24 hours. The cells to be tested were labeled with 51Cr or 3 H -TdR. The cells were cultured in a 96-well culture at a concentration of 1 × 10 5 / well Plate, the role of 4h gently washed off non-adherent cells. The radiosensitivity of 51Cr and 3 H -TdR labeled adherent cells was detected by the corresponding detection system, and its sensitivity and stability were compared. Results 51Cr release experiment and 3 H-TdR incorporation method all had good sensitivity and stability. In comparison, 3 H -TdR incorporation is more sensitive and stable. Both of these methods can accurately detect cell adhesion and reflect its relative strength. Conclusion The method of isotope labeling has good sensitivity and stability with less interference factors, and the results are stable and reliable. The sensitivity and stability of 3H-TdR incorporation are better than that of 51Cr.