利用树枝状PAMAM大分子对电极表面进行修饰,戊二醛偶联固定抗原,并将羧基化的多壁碳纳米管偶联上二抗和辣根过氧化物酶制备多酶标记二抗复合物做检测信号,构建了具有双重放大效应的苯并[a]芘免疫传感器。在最优实验条件下,基于竞争法对苯并[a]芘实现了较好的检测性能,其线性范围为0.05~1.2μmol/L,检出限为0.02μmol/L。方法可应用于水体中苯并[a]芘的检测。 The dendritic PAMAM macromolecule was used to modify the electrode surface, the glutaraldehyde coupling was used to immobilize the antigen, and the carboxylated multi-walled carbon nanotubes were coupled to the secondary antibody and horseradish peroxidase to prepare the multi-enzyme labeled secondary antibody complex To do the detection signal, we constructed the benzo [a] pyrene immunosensor with double amplification effect. Under optimal experimental conditions, the detection performance of benzo (a) pyrene was better than that of the competition method, with the linear range of 0.05 ~ 1.2μmol / L and the detection limit of 0.02μmol / L. The method can be applied to the detection of benzo [a] pyrene in water.