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AIM:To study the inhibitory effect of all-trans retinoic acidon human hepatocellular carcinoma cell line SMMC-7721and to explore the mechanism of its effect.METHODS:SMMC-7721 cells were divided into twogroups,one treated with all-trans retinoic acid (ATRA) for5 days and the other as a control group.Light microscopeand electron microscope were used to observe themorphological changes.Telomerase activity was analyzedwith silver-stained telomere repeated assay protocal(TRAP).Expression of Caspase-3 was demonstrated withwestern blot.RESULTS:ATRA-treated cells showed differentiationfeatures including small and pyknotic nuclei,densely stainedchromatin and fewer microvilli.Besides,ATRA could inhibitthe activity of telomerase,promote the expression ofCaspase-3 and its activation.CONCLUSION:Telomerase activity and Caspase-3expression are changed in human hepatocellular carcinomacell line SMMC-7721 treated with all-trans retinioc acid.The inhibition of telomerase activity and the activation ofCaspase-3 may be the key steps through which ATRAinhibits the proliferation of SMMC-7721 cell line.
AIM: To study the inhibitory effect of all-trans retinoic acid on human hepatocellular carcinoma cell line SMMC-7721 and to explore the mechanism of its effect. METHODS: SMMC-7721 cells were divided into twogroups, one treated with all-trans retinoic acid (ATRA ) for5 days and the other as a control group. Light microscope and electron microscope were used to observe themorphological changes. DNA activity was analyzed with silver-stained telomere repeated assay protocal (TRAP). Expression of Caspase-3 was demonstrated with western blot. -treated cells showed differentiation features including small and pyknotic nuclei, densely stained chromatin and fewer microvilli. Besides, ATRA could inhibit the activity of telomerase, promote the expression of Caspase-3 and its activation. CONCLUSION: Telomerase activity and Caspase-3 expression are changed in human hepatocellular carcinoma cell line SMMC-7721 treated with all-trans retinioc acid. The inhibition of telomerase activity and the activation ofCa spase-3 may be the key steps through which ATRA inhibits the proliferation of SMMC-7721 cell line.