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转基因大豆GTS40-3-2转化体是种植面积最广的转基因大豆品种,根据GTS40-3-2转化体特异性序列设计引物,应用可视化环介导等温扩增技术,对其进行快速扩增及可视化判断;同时建立转基因成分人工污染的食品模型,比较了LAMP法与定性PCR方法检测的敏感性。结果表明:该方法仅对GTS40-3-2转化体产生特异性扩增,灵敏度高达0.001%。所建立的GTS40-3-2转化体特异性现场可视化筛查方法具有较高的特异性与灵敏性,操作简单、快速,可用于转基因大豆GTS40-3-2成分污染的现场可视化检测。
Transgenic soybean GTS40-3-2 transformants are the most widely planted transgenic soybean varieties. Based on the specific sequence of GTS40-3-2 transformants, primers were designed, and visualized by ring-mediated isothermal amplification and rapid amplification Visual judgment. At the same time, a food contamination model of GMO was established, and the sensitivity of LAMP assay and qualitative PCR assay was compared. The results showed that this method only specific amplification of GTS40-3-2 transformants, the sensitivity of up to 0.001%. The established visualization method of GTS40-3-2 transformant is highly specific and sensitive, easy to operate and fast, and can be used for field visualization of the contamination of GTS40-3-2 genetically modified soybean.