论文部分内容阅读
目的与方法应用ＥＬＩＳＡ法研究了１８例癌性渗出液（ＭＯＦ）中内源性ＩＬ－２、ＩＬ－６、ＩＬ－８、ＩＦＮγ和ＴＦＮα的生物学活性，并与恶性肿瘤病人（ＭＴＤ）血清、正常人、结核性胸膜炎（ＴＢＰ）和肝硬化病人（ＣＲＳ）进行了比较分析。结果ＭＴＤ血清中ＩＬ－６活性高于与正常成人组（Ｐ＜０．０５）；ＩＬ－２和ＩＦＮγ活性亦低下（Ｐ＜０．５）。ＭＴＤ血清中ＩＬ－２、ＩＬ－６ＴＮＦα活性显著低于ＴＢＰ（Ｐ＜０．００１）；ＩＬ－８和ＩＦＮγ活性亦降低（Ｐ＜０．０５）。ＭＯＦ中的ＩＬ－６、ＩＦＮγ水平显著高于ＴＢＰ组（Ｐ＜０．０１；Ｐ＜０．０５）；ＩＬ－２却明显降低（Ｐ＜０．０５）。ＭＴＤ血清中ＩＬ－２、ＩＬ－６低于ＣＲＳ组（Ｐ＜０．０５）；ＭＯＦ中ＩＬ－６、ＩＮＦγ水平高于ＣＨＡＤ组（Ｐ＜０．００１；Ｐ＜０．０５）；ＩＬ－２和ＩＬ－８则低于ＣＲＳ组（Ｐ＜０．０５；Ｐ＜０．０１）。结论ＭＴＤ血清和ＭＯＦ中ＩＬ－２、ＩＬ－６、ＩＬ－８和ＩＦＮγ活性反映了ＭＴＤ抗肿瘤免疫的功能状态。ＩＬ－６和ＩＬ－８活性对于ＭＴＤ预后的估计具有重要的意义。
Objectives and methods To study the biological activities of endogenous IL-2, IL-6, IL-8, IFNγ and TFNα in 18 cancerous exudates (MOF) by ELISA, and to compare with patients with malignant tumor (MTD). Serum, normal, tuberculous pleurisy (TBP) and cirrhosis patients (CRS) were compared. Results The activity of IL-6 in serum of MTD was higher than that in normal adult group (P<0.05); the activity of IL-2 and IFNγ was also low (P<0.5). The activity of IL-2 and IL-6TNFα in serum of MTD was significantly lower than that of TBP (P<0.001); the activity of IL-8 and IFNγ was also decreased (P<0.05). The levels of IL-6 and IFNγ in MOF were significantly higher than those in TBP group (P<0.01; P<0.05); IL-2 was significantly decreased (P<0.05). IL-2 and IL-6 in MTD serum were lower than those in CRS group (P<0.05); IL-6 and INFγ levels in MOF were higher than those in CHAD group (P<0.001; P<0.05); IL- 2 and IL-8 were lower than those in CRS group (P<0.05; P<0.01). Conclusion The activity of IL-2, IL-6, IL-8 and IFNγ in MTD serum and MOF reflects the functional status of MTD antitumor immunity. The activity of IL-6 and IL-8 is of great significance for the estimation of the prognosis of MTD.