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目的探讨尿酸钠(monosodium urate,MSU)结晶体外刺激对多形核中性粒细胞(polymorphonuclear neutrophils,PMNs)、单核细胞细胞株THP-1、成纤维滑膜细胞(fibroblast-likes synoviocytes,FLSs)的活性及三种细胞趋化PMNs功能的影响。方法体外培养人PMNs、THP-1、FLSs,每种细胞各分为对照组和MSU组,并设无细胞的对照组和MSU组,对照组均为不含MSU结晶的培养液,MSU组为含终浓度为500 mg/L的MSU结晶培养液,所有细胞组于培养0、12、24、48 h后MTS法检测细胞的增殖,所有组别采用Transwell法观察培养24 h后对PMNs的趋化作用。结果培养12、24、48 h后,PMNs、THP-1的MSU组和对照组活性均随着时间的延长而增加,各时间点MSU组细胞活性均较对照组明显增高,比较差异具有统计学意义(P<0.01)。FLSs的MSU组在12、48 h细胞活性较对照组有明显降低(P<0.05),24 h时降低无明显差异(P>0.05)。培养24 h后,与对照组比较,PMNs和THP-1的MSU组对PMNs的趋化作用明显下降,差异有统计学意义(P<0.05),FLSs的MSU组对PMNs的趋化作用明显增加,差异具有统计学意义(P<0.05)。结论 MSU结晶在一定时间内,能明显促进PMNs、THP-1细胞活性增加,而对FLSs的活性则有抑制作用。MSU结晶刺激后PMNs、THP-1对PMNs的趋化下降,而FLSs对PMNs的趋化增加。
Objective To investigate the effects of monosodium urate (MSU) crystals on the expression of polymorphonuclear neutrophils (PMNs), monocyte cell line THP-1, fibroblast-like synoviocytes (FLSs) Activity and the function of three cell chemotactic PMNs. METHODS: Human PMNs, THP-1 and FLSs were cultured in vitro. Each cell was divided into control group and MSU group. Cell-free control group and MSU group were established. The control group were all MSU- MSU containing 500 mg / L final concentration of the culture medium, all cells in culture 0,12,24,48 h after MTS assay cell proliferation, all groups using the method of Transwell observed after 24 h culture PMNs tended to The role of. Results After 12, 24 and 48 h incubation, the activity of PMNs and THP-1 in MSU group and control group increased with time, and the cell viability in MSU group was significantly higher than that in control group at each time point, the difference was statistically significant Significance (P <0.01). The cell viability of MSU group was significantly lower than that of control group (P <0.05) at 12,48 h and no significant difference at 24 h (P> 0.05). Compared with the control group, the chemotactic effect of PMNs and THP-1 MSU group on PMNs was significantly decreased after 24 h incubation (P <0.05), and the chemotactic effect of PMNs was significantly increased in FLSs MSU group , The difference was statistically significant (P <0.05). Conclusion The crystallization of MSU can obviously promote the proliferation of PMNs and THP-1 cells in a certain period of time, but inhibit the activity of FLSs. PMNs and THP-1 decreased the chemotacticity of PMNs after MSU crystal stimulation, while the chemotactic effects of FLSs on PMNs increased.