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目的:观察滋阴泻火中药对辛基酚和双酚A染毒大鼠子宫、卵巢的雌激素受体(ER)、芳香化酶(AR)、胰岛素样生长因子(IGF-Ⅰ)基因和蛋白表达的影响,探讨中药拮抗环境内分泌干扰物(EEDS)拟雌激素活性的作用机制。方法:3周龄雌性SD大鼠染毒组给予辛基酚和双酚A联合喂饲,治疗组给予滋阴泻火中药与染毒物同时喂饲,对照组喂饲玉米油,每天灌胃1次,连续14天,分别检测子宫湿重及脏器系数,子宫、卵巢的ER、AR、IGF-Ⅰ基因和蛋白表达。结果:染毒组与对照组比较子宫湿重及脏器系数增加,子宫、卵巢ER、AR、IGF-Ⅰ基因和蛋白表达均上调,治疗组与染毒组比较,子宫湿重及脏器系数降低,子宫、卵巢ER、AR、IGF-Ⅰ基因和蛋白表达均下调。结论:EEDS对生殖器官具有显著的拟雌激素作用,而滋阴泻火中药EEDS对的拟雌激素活性具有拮抗作用,中药的治疗干预可使生殖器官ER、AR、IGF-Ⅰ的基因和蛋白表达显著下调,从而显著降低生殖器官对EEDS的敏感性,这可能是中药有效拮抗EEDS拟雌激素活性的作用机制。
OBJECTIVE: To observe the estrogen receptor (ER), aromatase (AR) and insulin-like growth factor (IGF-I) genes in the uterus and ovary of rats exposed to nourishing yin and purging fire for octylphenol and bisphenol A exposure. The effect of protein expression on the mechanism of action of Chinese herbs in antagonizing the estrogenic activity of environmental endocrine disruptors (EEDS). METHODS: Three-week-old female SD rats were administered with octylphenol and bisphenol A. The treatment group was given both yin and diarrhea and traditional Chinese medicine and poisons. The control group was fed with corn oil and was given daily. For 14 consecutive days, the uterine wet weight and organ coefficient, ER, AR, IGF-I gene and protein expression in uterus and ovary were measured. RESULTS: The uterine wet weight and organ coefficient were increased in the treated group compared with the control group, and the ER, AR, IGF-I gene and protein expression in the uterus and ovary were all increased. The wet weight of the uterus and organ coefficient were compared between the treated group and the treated group. Decreased, uterine, ovarian ER, AR, IGF-I gene and protein expression were downregulated. Conclusion: EEDS has significant estrogenic effects on genital organs, while EEDS of yin and diarrhea eliminates antagonism on the estrogenic activity of estrogens. The therapeutic intervention of traditional Chinese medicine can make genes and proteins of ER, AR and IGF-I in reproductive organs. The expression was significantly down-regulated, which significantly reduced the susceptibility of reproductive organs to EEDS. This may be the mechanism by which Chinese herbs effectively antagonize the estrogenic activity of EEDS.