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α_1肾上腺素受体(α_1-AR)是介导血管平滑肌收缩的主要受体,它在调节血压、血管阻力及器官血流量中发挥十分重要的作用.根据大鼠大脑皮层、海马、输精管及脾脏等组织对WB4101,5MU及(+)Niguldipine等α_1-AR 拮抗剂的亲和性和对氯乙基可乐啶(CEC)的敏感性,α_1-AR曾被分成α_(1A)及α_(1B)2种亚型.随后通过大量的药理学和分子生物学研究,现已发现α_1-AR含有3种亚型.它们分别为α_(1A),α_(1B)和α_(1D).并发现α_(1D)-AR的某些药理特性与α_(1A)-AR和α_(1B)-AR相似,并和α_(1B)-AR一样,可被CEC不可逆阻断.最近,Saussy等根据α_(1D)-AR的药理特性,首先提出去甲肾上腺素引起的大鼠主动脉的收缩是由α_(1D)受体亚型所介导的.但该研究仅在功能上显示了大鼠主动脉的α_i-AR亚型.由于血管组织与拮抗剂结合的高非特异性和缺乏区分α_(1)-AR 3种亚型的拮抗剂,很难采用放射配基结合实验来研究大鼠主动脉中α_1-AR 3种亚型的蛋白质水平的分布.Testa等曾经报道大鼠主动脉膜制品与CEC孵育后与α_1-AR拮抗剂哌唑嗪的结合位点下降88%.但这一结果并未回答α_(1D)与α_(1B)之间的比例.因此,大鼠主动脉中α_1-AR 3种亚型的分布比例尚未清楚.本文采用定量液相杂交方法测定α_1-AR 3种亚型的mRNA含量,试图从mRNA水平?
α_1-adrenoceptor (α_1-AR) is the main receptor mediating the contraction of vascular smooth muscle, which plays an important role in regulating blood pressure, vascular resistance and organ blood flow.According to the rat cerebral cortex, hippocampus, vas deferens and spleen (1A1), α1 (1B) and α1-AR (α1-AR) were detected by ELISA, 2 subtypes.Afterwards, a large number of pharmacological and molecular biological studies have been found, α_1-AR contains three subtypes, which are α 1A, α 1B and α 1 D, Some pharmacological properties of (1D) -AR are similar to that of α 1A 1A and-1B 1B, and are irreversibly blocked by CEC, as with α 1B 1B -AR. Recently, Saussy et al. 1D) -AR pharmacological properties, first proposed norepinephrine-induced contraction of the rat aorta is mediated by the α 1D receptor subtypes but this study only shows the function of rat aorta Of α_i-AR subtypes.Radial ligand binding experiments are very difficult to study in rats due to the high nonspecificity and lack of antagonism of vascular subtype α_ (1) -AR in vascular tissues and antagonists The distribution of protein levels in the α_1 -AR subtypes in the veins has been reported by Testa et al as a 88% reduction in the binding site for azone antagonist prazosin after incubation with CEC in rats However, the ratio between α 1 -D and α 1 B has not been answered.Therefore, the proportion of α 1 -AR 3 isoforms in rat aorta has not been clear yet.In this paper, quantitative determination of α 1-AR 3 Subtype mRNA levels, trying to level from mRNA?