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目的 建立驱虫斑鸠菊中异绿原酸A、异绿原酸C的含量测定方法.方法 Shim-pack VP-ODS C18柱(4.6 mm×250 mm,5 μm),甲醇-乙腈0.4%磷酸溶液(13.5∶13.5∶73)为流动相,流速:1 mL·min-1,柱温35 ℃,检测波长323 nm,进样量:10 μL.结果 异绿原酸A浓度在5.825~69.9 μg·mL-1与峰面积呈良好的线性关系,异绿原酸C浓度在5.15~61.80 μg·mL-1与峰面积呈良好的线性关系,二者加样回收率分别为98.70%~101.92%(RSD＝1.04%,n＝9)、95.99%~102.52%(RSD＝1.90%,n＝9).结论 该方法简便、快速、准确,可用于驱虫斑鸠菊中异绿原酸A、C含量测定及药材质量控制.“,”Objective To establish the chromatographic conditions of isochlorogenic acid A and isochlorogenic acid C in vernonia anthelmintica. Methods By changing the mobile phase,flow rate,column temperature and other chromatographic conditions,the best chromatographic conditions was we pursued to established. Results The linear relationship between the concentration of isochlorogenic acid A and the peak area was between 5. 825-69. 9 μg·mL-1, and the concentration of isochlorogenic acid C,was between 5.15-61.80 μg·mL-1and the peak area was good . The sample recovery rates of the two groups were 98.70%-101.92%(RSD＝1.04%,n＝9)、95.99%-102.52%(RSD＝1.90%,n＝9). Conclusion The method is simple,rapid, accurate and reliable for the determination of isochlorogenic acid A and isochlorogenic acid C in Vernonia anthelmintica and also for the quality control of the raw material.