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为了开展肠癌免疫学诊断的研究、本室就肠癌组织是否存在肿瘤特异性抗原或肿瘤相关抗原作了初步探讨。一、肠瘤组织提取液的制备:取手术切除新鲜肠癌组织,充分洗涤,除掉结缔组织和脂肪后,剪细,以冷PBS(pH7.2)在水浴中匀浆,于4℃离心(4,000转/30分钟),取上清经高速离心(35,000转/30分钟)后,去沉渣,测定上清液蛋白含量为18.3mg/ml,分装小瓶,低温冰冻保存备用。
In order to develop a study on the immunological diagnosis of colon cancer, we have conducted a preliminary study on the presence or absence of tumor-specific antigens or tumor-associated antigens in colon cancer tissues. First, the preparation of intestinal tumor tissue extract: freshly removed colorectal cancer tissue, fully washed, remove the connective tissue and fat, cut fine, with cold PBS (pH7.2) in a water bath, centrifuged at 4 °C (4,000 rpm/30 minutes), the supernatant was centrifuged at high speed (35,000 rpm for 30 minutes), sediment was removed, and the supernatant was assayed for protein content of 18.3 mg/ml. The vial was aliquoted and frozen at low temperature for later use.