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目的分析2015年镇江地区诺如病毒的感染状况及进化重组现象。方法收集2015年镇江地区哨点医院腹泻患者的粪便标本及突发疫情的病例标本,采用实时荧光定量PCR(q RT-PCR)方法进行诺如病毒GI/GII核酸检测,阳性样本采用逆转录PCR(RT-PCR)方法扩增诺如病毒基因的Rd Rp区和Capsid区,序列测定、基因分型,运用Mega软件和RDP软件进行序列进化和基因重组分析。结果 832份样本中诺如病毒阳性率为5.05%(42/832),GII组阳性率为4.81%(40/832),占95.24%,GII组在10~20岁的中青年中阳性率最高,达25.00%(14/56)。2015年诺如病毒的检出率以1月最高,11月、2月次之,以GII.17型所占比例最高为63.89%(23/36),新GII.17型变异株为最主要的流行株。主要的重组事件为诺如病毒GII.P12_GII.3型的镇江毒株ZJ111/2015/CHN和ZJ119/2015/CHN,是由加拿大的参考株U02030/Toronto/CA/GII.3和英国的参考株X86557/Lordsdale/UK/GII.4重组而成。结论 2015年镇江地区诺如病毒感染以GII组为主,中青年阳性率最高,新GII.17型变异株为最主要的流行基因型,主要的重组事件为GII.3与GII.4的重组。
Objective To analyze the infectious status and evolutionary recombination of Norovirus in Zhenjiang area in 2015. Methods Stool specimens and cases of sudden outbreaks of diarrhea patients in sentinel hospital of Zhenjiang area in 2015 were collected. Norovirus GI / GII DNA was detected by real-time fluorescence quantitative PCR (q RT-PCR). The positive samples were detected by reverse transcription PCR (RT-PCR) was used to amplify the RdRp and Capsid regions of Norovirus. The sequences were sequenced and genotypes were genotyped. Sequence evolution and gene recombination analysis were performed using Mega software and RDP software. Results The positive rate of Norovirus in 832 samples was 5.05% (42/832). The positive rate of GII group was 4.81% (40/832), accounting for 95.24%. The GII group had the highest positive rate in middle and young aged 10-20 years , Up 25.00% (14/56). The detection rate of norovirus in 2015 was the highest in January, followed by November and February, the highest proportion of GII.17 was 63.89% (23/36), and the new GII.17 variant was the most important Of the popular strain. The major recombination event was the strains of Zhenjiang Strain ZJ111 / 2015 / CHN and ZJ119 / 2015 / CHN of norovirus GII.P12_GII.3 from Canada reference strain U02030 / Toronto / CA / GII.3 and the UK reference strain X86557 / Lordsdale / UK / GII.4 reorganized. CONCLUSION: Norovirus infection in Zhenjiang area was mainly GII group in 2015 with the highest positive rate in middle-aged and young people. The new GII.17 variant was the most prevalent genotype. The main recombination events were recombination of GII.3 and GII.4 .