通过RT-PCR方法和基因克隆技术对黄早四Rbx1基因进行分子克隆和序列分析,构建黄早四Rbx1的原核表达系统,成功表达出带有His标签的黄早四Rbx1融合蛋白,并进一步利用His抗体,用蛋白免疫印迹的方法对重组蛋白进行鉴定。结果表明,重组蛋白能够与His抗体发生免疫反应,验证了黄早四Rbx1在原核表达系统中成功表达。 The Rbx1 gene of Huangzaozao was cloned and sequenced by RT-PCR and gene cloning techniques. The prokaryotic expression system of Rbx1 was constructed, and the His-tagged RAS-Rbx1 fusion protein was successfully expressed and further utilized His antibody was used to identify the recombinant protein by Western blotting. The results showed that the recombinant protein was able to immunoreact with His antibody, which proved that Rbx1 was successfully expressed in prokaryotic expression system.