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目的探讨十二碳烯酸对人H4细胞的毒性作用机制。方法采用MTT法、流式细胞仪检测十二碳烯酸对人H4细胞的毒性作用。结果分别给予人H4细胞0.3,0.9,2.7 mg·L-1十二碳烯酸培养48 h,十二碳烯酸对人H4细胞增殖抑制率分别为41.64%,70.97%,81.52%,半数抑制浓度(IC50)为0.40 mg·L-1,与对照组相比,差异有统计学意义(P<0.01);凋亡率分别为10.16%,18.22%,24.06%。结论十二碳烯酸对人H4细胞具有显著的毒性作用,其机制可能与损伤线粒体结构,抑制细胞分裂等有关。
Objective To investigate the toxic mechanism of dodecenoic acid on human H4 cells. Methods MTT assay was used to detect the toxic effects of dodecenoic acid on human H4 cells by flow cytometry. Results The inhibitory rates of dodecenoic acid on the proliferation of human H4 cells were 41.64%, 70.97%, 81.52%, respectively, and half of them were inhibited by 0.3,0.9,2.7 mg · L-1 dodecenoic acid for 48 h. (IC50) was 0.40 mg · L-1, which was significantly different from the control group (P <0.01). The apoptosis rates were 10.16%, 18.22% and 24.06% respectively. Conclusion Dodecenoic acid has a significant toxic effect on human H4 cells, which may be related to the damage of mitochondrial structure and inhibition of cell division.