论文部分内容阅读
采用聚丙烯酰胺凝胶电泳技术,分析了兰州鲇(Silurus lanzhouensis)肌肉、心脏、肝脏、脑、脾脏、肾脏和眼睛等7种组织中乳酸脱氢酶(LDH)、酯酶(EST)、苹果酸脱氢酶(MDH)、醇脱氢酶(ADH)、山梨醇脱氢酶(SDH)和谷氨酸脱氢酶(GDH)等6种同工酶的表达模式,并对各同工酶的酶谱进行了分析。结果表明:这6种同工酶在兰州鲇7种组织中的分布均存在明显的组织特异性;6种酶共记录出17个基因位点,其中EST-2、EST-3、MDH-3和SDH-2为多态位点;兰州鲇群体的多态位点比例P为23.53%,位点平均有效等位基因数Ne为1.24;平均观测杂合度和平均期望杂合度分别为0.137 2和0.105 1,Hardy-Weinberg遗传偏离指数D为0.305 4,杂合子处于过剩状态。从以上数据中可以看出,兰州鲇野生群体遗传变异程度较大,但是有效等位基因数偏低,有必要对兰州鲇野生种质资源及时加以保护。
Polyacrylamide gel electrophoresis was used to analyze the effects of LDH, EST, (MDH), alcohol dehydrogenase (ADH), sorbitol dehydrogenase (SDH) and glutamate dehydrogenase (GDH) The zymogram was analyzed. The results showed that there were obvious tissue-specific distributions of these six isozymes in seven tissues of Lanzhou chrysanthemum; sixteen kinds of enzymes recorded a total of 17 loci, of which EST-2, EST-3, MDH-3 And SDH-2 were polymorphic loci. The proportion P of polymorphic loci in Lanzhou population was 23.53%, the average number of effective alleles at loci was 1.24. The average observed heterozygosity and average expected heterozygosity were 0.137 2 and 0.105 1, Hardy-Weinberg genetic divergence index D is 0.305 4, Heterozygotes in excess state. It can be seen from the above data that there is a large degree of genetic variation among the wild populations in the population of Lanzhou, but the number of effective alleles is low, so it is necessary to protect wild germplasm resources of Lanzhou in time.