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目的 研究番泻叶提取物对游离的豚鼠结肠平滑肌细胞收缩的影响 .方法用含 1 g· L-1胶原酶及 0 .1 g· L-1大豆胰蛋白酶抑制剂的消化液消化 ,获得游离的单个豚鼠结肠平滑肌细胞 ;将不同浓度番泻叶提取物加入细胞悬液中 ,用测微尺观察随机遇到的 50个细胞长轴变化情况 ,计算用药前后细胞平均长度 .结果 成功获得了游离的结肠平滑肌细胞 .证实不同浓度的番泻叶提取物 (0 .2 2 ,0 .44,0 .89g· L-1 )与平滑肌细胞共孵育后 ,可使细胞的平均长度缩短为 (93±2 0 ) ,(86± 2 5) ,(85± 1 5)μm,生理盐水对照组为 (1 0 2± 2 3)μm,细胞长度下降的百分数为 8.7% ,1 5.7%和 1 6.6% ,与对照组相比有显著差异 (P<0 .0 5) ;在剂量为 0 .2 2～ 0 .89g·L-1 范围内 ,番泻叶对平滑肌细胞的收缩作用逐渐增强 (P<0 .0 5) ,呈剂量 -效应关系 .结论 番泻叶提取物对豚鼠结肠平滑肌细胞有直接的收缩作用
Objective To study the effect of senna extract on the contraction of free guinea pig colonic smooth muscle cells. Methods Digestion with digestive fluid containing 1 g·L-1 collagenase and 0.1 g·L-1 soybean trypsin inhibitor was performed to obtain free A single guinea pig colon smooth muscle cells; different concentrations of senna extract added to the cell suspension, using a micrometer to observe the changes in the long axis of 50 randomly encountered cells, calculate the average length of cells before and after treatment. The results were successfully obtained Colonic Smooth Muscle Cells. It was demonstrated that co-incubation of smooth muscle cells with different concentrations of senna extract (0.22, 0.44 g·L-1) resulted in a shortened average cell length of (93±). 2 0), (86 ± 25), (85 ± 1 5) μm, normal saline control group (1 0 2 ± 2 3) μm, percentage of cell length decrease was 8.7%, 15.7%, and 16.6% Compared with the control group, there was a significant difference (P<0.05). In the dose range of 0.22 to 0.89 g·L-1, the contraction effect of senna on smooth muscle cells gradually increased (P< 0. 0 5) , showing a dose-effect relationship. Conclusion Senna extract has a direct contraction effect on guinea pig colonic smooth muscle cells.