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为研究中药复方软肝汤对CCl4诱导的大鼠肝纤维化肝组织中表达TGFβ1、Smad3和Smad7的影响,探讨软肝汤预处理可能的抗纤维化机制。本研究将60只Wistar大鼠被随机分成对照组、模型组、复方软肝汤预处理组及鳖甲软肝片组,其中复方软肝汤预处理组又分为小剂量组、常规剂量组和大剂量组,肝纤维化模型由大鼠背部皮下注射CCl4构建,分别用HE和VG染色法染色,肝纤维化程度在光学显微镜下观测。同时采用SABC免疫组化方法检测各实验组TGF-β1、Smad3和Smad7的表达情况。结果显示:和对照组相比,TGF-β1、Smad3在模型组中表达增强明显,而Smad7在模型组中的表达显著下降(P<0.05)。复方软肝汤预处理各组中大鼠肝组织中TGF-β1表达和模型组相比均有减弱(P<0.05),且随着复方软肝汤剂量的增加而减少;复方软肝汤随着剂量增加逐步下调Smad3的表达(P<0.05),随着复方软肝汤剂量增加上调Smad7的表达(P<0.05)。另外,复方软肝汤组大鼠肝脏病理变化显著改善;鳖甲软肝片组与模型组比较,TGF-β1、Smad3表达减弱;Smad7表达增加(P<0.05)。由此可知,复方软肝汤后处理对大鼠肝组织TGF-β1/Smad信号通路有明显的影响,随着剂量的增加抑制CCl4诱导的大鼠肝纤维化的作用逐步增强,其作用机制可能为抑制TGF-β1表达,下调Smad3及上调Smad7的表达。
To study the effect of RuanNanTang decoction on the expression of TGFβ1, Smad3 and Smad7 in CCl4-induced hepatic fibrosis in rats and to explore the possible anti-fibrosis mechanism of RuanGan decoction pretreatment. In this study, 60 Wistar rats were randomly divided into control group, model group, Fufang RuanGanTang pretreatment group and BieJiaRanGanPian group. FuLanRuanTan pretreatment group was divided into small dose group, conventional dose group And high-dose group. The hepatic fibrosis model was constructed by subcutaneous injection of CCl4 on the back of rats. The liver fibrosis was stained with HE and VG staining, and the degree of hepatic fibrosis was observed under a light microscope. SABC immunohistochemistry was used to detect the expression of TGF-β1, Smad3 and Smad7 in each experimental group. The results showed that compared with the control group, the expression of TGF-β1 and Smad3 increased significantly in the model group, while the expression of Smad7 in the model group decreased significantly (P <0.05). Compared with the model group, the expression of TGF-β1 in the compound Ruan-Tang decoction group was weaker than that in the model group (P <0.05), and decreased with the increase of compound Ruan-yuan decoction The dose of Smad3 was down-regulated (P <0.05), and the expression of Smad7 was up-regulated with the increase of Fufang Ruangan decoction (P <0.05). In addition, the pathological changes of the liver of the compound RuanGanTang group were significantly improved. Compared with the model group, the expression of TGF-β1 and Smad3 in the BieJiaRanGanTian group was decreased and the expression of Smad7 was increased (P <0.05). It can be seen that the compound Ruan Tang decoction has a significant effect on the TGF-β1 / Smad signaling pathway in rat liver tissue, and the effect of inhibiting CCl4-induced hepatic fibrosis gradually increases with the increase of dose, and its mechanism may be To inhibit the expression of TGF-β1, down-regulate Smad3 and up-regulate the expression of Smad7.