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目的检测食管鳞癌组织中神经轴突导向因子(Netrin-1)及mRNA表达。方法应用免疫组化SP法和原位杂交方法检测50例食管鳞癌组织及其相应的19例癌旁不典型增生组织和20例正常食管黏膜组织中Netrin-1、mRNA的表达。结果①食管鳞癌组织中Netrin-1、mRNA阳性表达率均高于癌旁不典型增生组织和正常食管黏膜组织(P均<0.05)。②有淋巴结转移组食管鳞癌组织中Netrin-1、mRNA阳性表达率均高于无淋巴结转移组(P均<0.05)。结论人食管鳞癌组织中Netrin-1蛋白及mRNA均呈高表达,可能参与食管癌的发生、发展。
Objective To detect the expression of Netrin-1 and mRNA in esophageal squamous cell carcinoma tissues. Methods Immunohistochemical SP method and in situ hybridization method were used to detect the expression of Netrin-1 and mRNA in 50 cases of esophageal squamous cell carcinoma and its corresponding 19 cases of adjacent atypical hyperplasia tissues and 20 cases of normal esophageal mucosa tissues. Results ① The positive expression rates of Netrin-1 and mRNA in esophageal squamous cell carcinoma were significantly higher than those in atypical hyperplasia tissues and normal esophageal mucosa (all P <0.05). ② The positive rate of Netrin-1 and mRNA expression in esophageal squamous cell carcinoma with lymph node metastasis were significantly higher than those without lymph node metastasis (all P <0.05). Conclusion The expression of Netrin-1 protein and mRNA in esophageal squamous cell carcinoma tissues is highly expressed, which may be involved in the occurrence and development of esophageal cancer.