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目的探讨ICAM-1和STAT4在肝癌组织中的表达情况及其与肿瘤TNM分期、血管侵犯、肿瘤大小及肿瘤分级的相互关系。方法选择2014年5月到2016年5月在我院及南昌大学第一附属医院普外科接受外科手术切除的60例肝癌患者肝细胞癌(HCC)组织和相应邻近非癌肝组织(ANLT)新鲜标本,HCC组织为实验组,ANLT组织为对照组。采用免疫组织化学法检测ICAM-1和STAT4在HCC和ANLT中的表达水平,比较ICAM-1和STAT4与患者TNM分期、侵袭性的关系。结果实验组中ICAM-1(80.00%vs 25.00%)表达阳性率显著高于对照组,STAT4表达阳性率(33.33%vs 73.33%)显著低于对照组(P<0.05)。侵袭性肝癌患者HCC组织中ICAM-1表达阳性率显著高于非侵袭肝癌患者(92.31%vs 70.59%,P<0.05);侵袭性肝癌患者HCC组织中STAT4表达阳性率显著低于非侵袭性肝癌患者(15.00%vs 42.50%,P<0.05)。Ⅲ期和Ⅳ期患者HCC组织中ICAM-1表达阳性率显著高于Ⅰ期和Ⅱ期患者(P<0.05),Ⅲ期和Ⅳ期、Ⅰ期和Ⅱ期ICAM-1表达阳性率差异不显著(P>0.05)。Ⅲ期和Ⅳ期患者HCC组织中STAT4阳性表达率显著低于Ⅰ期(P<0.05),Ⅲ期和患者HCC组织中STAT4阳性表达率显著低于Ⅱ期(P<0.05)。Ⅲ期和Ⅳ期患者HCC组织中STAT4阳性表达率差异不显著(P>0.05)。结论 ICAM-1和STAT4是肝细胞癌进展和侵袭的重要作用因子,可将其作为判断肝癌进展和转移的推测指标加以利用。
Objective To investigate the expression of ICAM-1 and STAT4 in hepatocellular carcinoma (HCC) and their relationships with tumor TNM staging, vascular invasion, tumor size and tumor grade. Methods Sixty patients with hepatocellular carcinoma (HCC) and corresponding adjacent non-cancerous liver tissue (ANLT) who underwent surgical resection in our hospital and the First Affiliated Hospital of Nanchang University from May 2014 to May 2016 were selected as fresh Specimens, HCC tissue as experimental group, ANLT tissue as control group. The expression of ICAM-1 and STAT4 in HCC and ANLT were detected by immunohistochemistry. The relationship between ICAM-1, STAT4 and TNM staging and invasiveness was compared. Results The positive rate of ICAM-1 (80.00% vs 25.00%) in the experimental group was significantly higher than that in the control group. The positive rate of STAT4 expression in the experimental group (33.33% vs 73.33%) was significantly lower than that in the control group (P <0.05). The positive rate of ICAM-1 in HCC tissues of patients with invasive liver cancer was significantly higher than that of non-invasive liver cancer patients (92.31% vs 70.59%, P <0.05). The positive rate of STAT4 in HCC tissues of patients with aggressive liver cancer was significantly lower than that of non-invasive liver cancer Patients (15.00% vs 42.50%, P <0.05). The positive rates of ICAM-1 in stage Ⅲ and stage Ⅳ were significantly higher than those in stage Ⅰ and stage Ⅱ (P <0.05). The positive rates of ICAM-1 in stages Ⅰ and Ⅱ were not significantly different between stage Ⅲ and stage Ⅳ (P> 0.05). The positive expression rate of STAT4 in stage Ⅲ and stage Ⅳ was significantly lower than that in stage Ⅰ (P <0.05). The positive expression rate of STAT4 in stage Ⅲ and in patients with HCC was significantly lower than that in stage Ⅱ (P <0.05). There was no significant difference in the expression of STAT4 in stage Ⅲ and stage Ⅳ HCC (P> 0.05). Conclusions ICAM-1 and STAT4 are important factors in the progression and invasion of hepatocellular carcinoma, which can be used as the predictors of the progress and metastasis of hepatocellular carcinoma.