利 用荧光探 针 Ｅ Ｂ（溴 化乙啶）研 究茶多酚 （ Ｔ Ｐ）与 Ｄ Ｎ Ａ 的相 互作用 及顺 铂（ Ｃ Ｄ Ｄ Ｐ）对其 的影响，结果表明：在 Ｅ Ｂ Ｄ Ｎ Ａ 体系中加 入 Ｔ Ｐ 后，荧光光谱 形状和峰位几乎 无变化，而荧光强度 明显下降； Ｔ Ｐ 压低 Ｅ Ｂ Ｄ Ｎ Ａ 荧 光强度的效率 Ｄ 与其浓度成正相关，高浓度 Ｔ Ｐ 可以完全抑制 Ｅ Ｂ Ｄ Ｎ Ａ 荧光强度⒚ Ｔ Ｐ与 Ｃ Ｄ Ｄ Ｐ 对 Ｅ Ｂ Ｄ Ｎ Ａ 荧光压低作用存在叠加现象，表明 Ｔ Ｐ 可增强 Ｃ Ｄ Ｄ Ｐ 的药用效果，又 可降低其副作用⒚ Ｔ Ｐ使 Ｅ Ｂ Ｄ Ｎ Ａ 体系的荧 光偏振度 Ｐ增加，而荧光寿命 τ缩短，这表明 Ｔ Ｐ 与 Ｄ Ｎ Ａ 作用的机理可能是 Ｔ Ｐ 插 入了 Ｄ Ｎ Ａ 分子碱 基对平 面中，影 响了 Ｄ Ｎ Ａ 分子双 链的有 序度，并 使从 Ｄ Ｎ Ａ 分子中 向 Ｅ Ｂ 分子传递的能量减少⒚ Fluorescence probe E B (ethidium bromide) was used to study the interaction between tea polyphenols (T P) and D N A and the effect of cisplatin (C D D P) on it. The results showed that in E B D N A system after adding T P, the fluorescence spectrum shape and peak position almost no change, while the fluorescence intensity decreased significantly; T P down E B D N A fluorescence intensity of the efficiency D and its concentration is positively related to the high concentration of T P can be completely inhibited E B D N A fluorescent intensity ⒚ T P and C D D P on E B D N fluorescence under the effect of the superimposed phenomenon, indicating that T P can enhance the C D D P medicinal effect, but also reduce the side effects of ⒚ T P makes the fluorescence polarization degree P of E BD N A system increase and the fluorescence lifetime τ shortens, which indicates that the mechanism of action of T P and D N A may be that T P is inserted into the base-pair plane of D N A molecule, Affect the degree of orderliness of the double strand of the D N A molecule and shift from the D N A molecule toward E B Energy transfer is reduced sub ⒚