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对睾酮及表睾酮的三甲基硅烷化进行了详细考察,找到了较好的抗氧剂巯基乙醇,确定了较好的衍生化条件,衍生化产物单一。并采用GC—MS法测定了尿中睾酮与表睾酮的比值。实验条件为:以氦为载气,SE—54熔融石英柔性毛细管柱、程序升温进行样品分离,多离子检测(MID),监测m/z432的离子。该法专属、灵敏、快速。睾酮与表睾酮比值在1:1～10:1(睾酮为20ng/μl)与相应峰面积比呈线性关系(r=0.998),最低检测限为1ng,最低检测尿药浓度为8ng/ml。
The test of testosterone and epostereone trimethylsilylation were investigated in detail to find a better antioxidant mercaptoethanol, to determine a better derivatization conditions, derivatization of a single product. The ratio of testosterone in urine to epicastone testosterone was determined by GC-MS. The experimental conditions were: SE-54 fused silica capillary column with helium as carrier gas, temperature programmed sample separation, multi-ion detection (MID) and monitoring of m / z 432 ions. The law is exclusive, sensitive and fast. The ratio of testosterone to epicardia was linearly proportional to the peak area ratio (r = 0.998) at a ratio of 1: 1 to 10: 1 (testosterone 20 ng / μl), with a minimum detectable limit of 1 ng and a minimum urinary concentration of 8 ng / ml.