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利用花粉管通道法将辣椒总DNA导入小麦,获得T_0代种子。T_1代播种后表型总变异率为21.3%,对照组为4%;变异谱有9种,对照组只有2种。无论在变异谱上还是变异率上,经辣椒总DNA转化的小麦材料与对照组有着明显的差异。随机选取转化材料中的47株材料主茎旗叶(尖部1/4),提取DNA进行IRAP分析,共扩增条带6条,有5条存在多态性。花粉管通道法向小麦中导入远缘辣椒总DNA能够在当代引起多种变异,并且也可以在分子水平上检测到基因组的差异。进一步还要测定单株麦粒的Vc含量,并且对变异材料进行遗传情况评估,以期得到稳定遗传的、新的小麦种质资源。
Pollen tube pathway was used to introduce the total DNA of pepper into wheat to obtain T_0 generation seeds. The total phenotypic variation of T_1 was 21.3% after sowing, compared with 4% in the control group, 9 in the variation spectrum and only 2 in the control group. There was a significant difference between wheat material transformed with total DNA of pepper and control group, both in terms of mutation rate and mutation rate. Forty-seven materials were randomly selected from the flag leaf (1/4 of the tips) of the stems of the transformation materials. DNA was extracted and analyzed by IRAP. A total of 6 bands were amplified and 5 of them were polymorphic. The introduction of distant pepper total DNA into pollen tube pathway wheat can cause many variations in the contemporary world and also detect the differences in genomes at the molecular level. Furthermore, the Vc content of single grain was measured, and the genetic evaluation of the mutant material was carried out to obtain a stable and inherited new wheat germplasm resource.