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利用60Co-γ射线辐照引发,在聚苯乙烯(PS)微孔板表面接枝了聚丙烯酰胺(PAM)。傅里叶变换红外光谱(ATR-FTIR)与X射线光电子衍射(XPS)证明聚丙烯酰胺已成功接枝在聚苯乙烯表面。水接触角与吸光度测量表明PS-PAM微孔板表面性质有效地发生了变化。结果表明:8 k Gy辐照剂量、单体浓度2%,此时多孔板在450 nm波长处吸光度提高11%,蛋白吸附能力提高22.5%。
Poly (acrylamide) (PAM) was grafted on the surface of polystyrene (PS) microplates by irradiation with 60Co-γ radiation. Fourier Transform Infrared Spectroscopy (ATR-FTIR) and X-ray Photoelectron Diffraction (XPS) demonstrated that polyacrylamide was successfully grafted on polystyrene surface. Water contact angle and absorbance measurements showed that the surface properties of PS-PAM microplates effectively changed. The results showed that the irradiation dose of 8 k Gy and monomer concentration of 2%, the absorbency of porous plate increased 11% at 450 nm and the protein adsorption capacity increased 22.5%.