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本工作采用Ｃａ３（ＰＯ４）２ＤＮＡ共沉淀法，将含有编码ＳＶ４０病毒早期基因的ｐＳＧ５质粒转染原代培养的人甲状腺上皮细胞。在重复进行的１２次转染中，有１次细胞生存达２００ｄ，传了２６代。检测证实，转染后的细胞仍能保持少量Ｔ４的分泌和Ｔｇ的合成；细胞经长期培养后形态无异常，Ｇ带染色正常，对裸鼠无致瘤性。甲状腺滤泡上皮细胞长期培养模型的建立，为进一步研究细胞免疫在自身免疫性甲状腺炎中的作用打下了基础。
This work using Ca3 (PO4) 2DNA co-precipitation method, will contain the gene encoding SV40 early gene pSG5 plasmid transfected primary cultured human thyroid epithelial cells. In the repeated 12 transfection, there is a cell survival of 200d, passed 26 generations. Test confirmed that the transfected cells can still maintain a small amount of T4 secretion and Tg synthesis; cells after long-term culture without abnormal morphology, G-band staining normal, non-tumorigenic nude mice. The establishment of a long-term culture model of thyroid follicular epithelial cells lays the foundation for further study on the role of cellular immunity in autoimmune thyroiditis.