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本文在孵育的离体大鼠脊髓组织切片上，观察牛磺酸对缺氧／复氧损伤的影响。缺氧／复氧损伤引起大鼠脊髓组织ＡＴＰ含量减少和ＬＤＨ漏出增多，ＮＯ生成和ＮＯＳ活性明显增加。Ｌ精氨酸转运Ｖｍａｘ增加９７％（Ｐ＜００１），Ｋｍ值无明显变化。用１０和２０ｍｍｏｌ／Ｌ牛磺酸孵育与单纯缺氧／复氧组比较，显著减轻了缺氧／复氧引起的组织ＡＴＰ减少和ＬＤＨ漏出，ＮＯ生成分别低３４％（Ｐ＜００１）和４０％（Ｐ＜００１），组织Ｌ精氨酸转运Ｖｍａｘ降低３２％（Ｐ＜００１）和３８％（Ｐ＜００１），但仅轻度抑制ＮＯＳ活性增加。提示牛磺酸对Ｌ精氨酸／一氧化氮系统的影响可能是其对缺氧／复氧损伤的神经保护作用的机理之一。
In this paper, the excised rat spinal cord tissue sections to observe the impact of taurine on hypoxia / reoxygenation injury. Hypoxia / reoxygenation injury caused a decrease of ATP content and LDH leakage in spinal cord tissue of rats, NO generation and NOS activity increased significantly. L arginine transport increased Vmax 97% (P <0 01), Km no significant change. Incubation with 10 and 20 mmol / L taurine significantly reduced tissue ATP depletion and LDH leakage caused by hypoxia / reoxygenation compared with those in the hypoxia / reoxygenation group, respectively, with a 34% lower NO production (P <001) (P <001) and 38% (P <001), respectively, but only slightly inhibited the increase of NOS activity. It is suggested that the effect of taurine on L-arginine / nitric oxide system may be one of the mechanisms of its neuroprotective effect on hypoxia / reoxygenation injury.