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目的:研究前胡丙素(PraC)的心脏负性肌力作用的机制.方法:使用Ca2+敏感的荧光指示剂Fura2AM,在培养的新生鼠心肌细胞测定细胞内Ca2+的瞬间变化.结果:PraC10、30μmol·L-1引起[Ca2+]i瞬间变化最大值明显减少.PraC10,30μmol·L-1能部分抑制CaCl248mmol·L-1及Bayk8644100nmol·L-1增加的[Ca2+]i瞬间变化的峰值,但对[Ca2+]i瞬间变化的基线水平没有明显的影响.Ryanodine3μmol·L-1使[Ca2+]i瞬间变化的幅度明显降低,在此基础上,PraC30μmol·L-1继续抑制[Ca2+]i的瞬间变化.结论:PraC在完整心肌的负性肌力作用可能与其部分抑制兴奋收缩偶联过程中的Ca2+内流有关.
Objective: To study the mechanism of the negative inotropic effect of PraC (PraC). Methods: Ca2 + sensitive fluorescent indicator Fura2 AM, in cultured neonatal rat cardiomyocytes measured intracellular Ca2 + transient changes. Results: The maximum instantaneous change of [Ca2 +] i induced by PraC10, 30μmol·L1 was significantly decreased. PraC10, 30μmol·L-1 could partially inhibit the peak of [Ca2 +] i transiently increased in CaCl24.8mmol·L-1 and Bayk8644100nmol·L-1, but there was no obvious change in the baseline level of [Ca2 +] i influences. Ryanodine 3μmol·L-1 [Ca2 +] i instantaneous changes in the magnitude of significantly reduced, on this basis, Pra 30μmol · L-1 continue to inhibit [Ca2] i transient changes. Conclusion: The negative inotropic effect of PraC in intact myocardium may be related to its partial inhibition of Ca2 + influx during excitatory-contractile coupling.