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以MT为基本培养基并附加各种植物激素和生理活性物质的一系列培养基中,8周龄的“华盛顿脐橙”(Citrus sinensis Osbeck)未受精胚珠,通过体细胞胚胎发生或器官发生再生出完整植株。外源激素的种类和组合对愈伤组织的诱导、生长状况和分化有显著的影响。指示植物鉴定证明,已从感病母株中获得了脱去裂皮病和衰退病的无病毒珠心苗。
Eight-week-old “Citrus sinensis Osbeck” unfertilized ovules were regenerated by somatic embryogenesis or organogenesis in a series of media with MT as a basal medium supplemented with various plant hormones and physiologically active substances Complete plant. The types and combinations of exogenous hormones have a significant effect on the induction, growth and differentiation of callus. Indication of plant identification indicates that the virus-free Pearl seedling has been obtained from susceptible mother plants to remove split and recession.