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以发光菌青海弧菌(Vibrio qinghaiense sp.-Q67)为指示生物,4种农药包括残杀威、甲霜灵、多果定和西草净为研究对象,应用均匀试验设计法设计农药的四元混合物体系,共7条射线,应用微板毒性测试方法(MTA)系统测定4种农药及其四元混合物体系对Q67的发光抑制毒性,采用非线性最小二乘法拟合浓度-效应数据,以浓度加和模型(CA)为标准加和参考模型分析混合物毒性相互作用。结果表明,Logit或Weibull函数可有效地拟合4种农药的浓度–效应数据,其相关系数R>0.99,均方根误差RMSE<0.022;4种农药对发光菌Q67的毒性大小顺序为多果定(p EC50=5.35)>西草净(p EC50=3.56)>甲霜灵(p EC50=3.18)>残杀威(p EC50=3.00),其中多果定毒性最大,残杀威、甲霜灵和西草净对Q67的毒性比较接近;四元农药混合物体系的7条射线对Q67的毒性(p EC50值)与组分西草净和多果定的浓度比尤其是西草净和多果定的浓度比之和具有良好的正相关关系(R=0.8729,RMSE=0.076),但与残杀威和甲霜灵的浓度比之和具有良好的负相关关系(R=0.8729,RMSE=0.076);CA模型能很好地评估四种农药的四元混合物体系对Q67的毒性,即混合物体系呈经典的加和作用。
The four kinds of pesticides, including Propoxur, Metalaxyl, Dorocididin and Sai Cao Jing, were selected as the indicator organisms by the light-emitting bacteria Vibrio qinghaiense sp. -Q67. Mixture system, a total of seven rays, the use of microplate toxicity test (MTA) system for the determination of four pesticides and quaternary mixtures of Q67 luminescence inhibition of toxicity using non-linear least squares fitting concentration-response data to the concentration The additive model (CA) analyzes the mixture toxicity interactions for the standard plus reference model. The results showed that the Logit or Weibull function could effectively fit the concentration-response data of four pesticides with the correlation coefficient R> 0.99 and the root mean square error RMSE <0.022. The toxicity of four pesticides to Q67 was more than that of Q67 Metoclopramide (pEC50 = 3.18)> Metoclopramide (pEC50 = 3.18)> Propoxurium (pEC50 = 5.35) The toxicity to Q67 was close to that of simethicone. The toxicity of Q.7 (p EC50 value) to Q67 of the quaternary pesticide mixture system was significantly lower than that of simethicone and fructose (R = 0.8729, RMSE = 0.076), but had a good negative correlation with the sum of propoxur and metalaxyl concentrations (R = 0.8729, RMSE = 0.076) ; The CA model can well evaluate the toxicity of quaternary mixtures of four pesticides to Q67, that is, the mixture system is a classical additive effect.