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目的检测辽宁省长角血蜱中弗朗西斯菌属及其内共生体的携带情况。方法 2013年7月,在辽宁省采集长角血蜱357只共分成38组,进行土拉弗朗西斯菌(土拉菌)的特异培养和弗朗西斯菌属及其共生体16S r RNA的巢式PCR检测。对有目的片段扩增的PCR产物进行测序和同源性比对,并与网上已公布的弗朗西斯菌属和弗朗西斯菌属(类)内共生体的序列进行系统进化分析。结果有测序结果的13组蜱中,5组为土拉菌,8组为弗朗西斯菌属(类)内共生体。由此推算,河栏镇长角血蜱对于土拉菌的最小携带率为2.12%。系统进化分析显示,5组蜱和土拉菌聚在一个分支上,其余8组和弗朗西斯菌属(类)内共生体聚在一个主干分支上。结论首次发现辽宁省长角血蜱携带土拉菌及弗朗西斯菌属(类)内共生体,对于提醒当地卫生部门做好土拉热防控具有一定意义。
Objective To detect the presence of Francisella and its symbiont in L. longitianum. Methods In July 2013, 357 H. longicornis collected in Liaoning Province were divided into 38 groups, and the specific culture of Francisella tulara (Tulara) and nested PCR of Francisella and its symbiont 16S rRNA were performed . The PCR products amplified by PCR were sequenced and homologous compared with the published sequence of the symbionts within the family of Francisella and Francisella that were published online. Results Among the 13 ticks sequenced, 5 were Tulara, and 8 were Francisella symbionts. Based on this calculation, the minimum carriage rate of Toxoplasma gondii to Heteropneumoniae was 2.12%. Phylogenetic analysis showed that five groups of ticks and Tulara clustered on one branch, and the remaining eight groups clustered with a Francisella symbiont within a single trunk branch. Conclusions It was first found that P. longicornis carries Tetragenococcus and Francisella (type) endosymbionts, which is of great significance to remind the local health department to do TURP.